Bravo Yogurt and Non-Dairy Drink eliminate toxins from weight loss

From Dr. Ruggiero:

Attached, please find a short presentation explaining the risk of toxicity associated with weight loss in today’s polluted world and the benefits of Bravo as a means to detoxify while loosing weight.  Please feel free to use, share and distribute this presentation.

This presentation was given, booked as a “premiere event” at the  “Toxicity and Longevity Summit” in Chicago on October 26.    The lead researcher,  Dr. Jerry Blythe and myself (Marco Ruggiero) demonstrated how weight loss protocols accompanied by Bravo lead to elimination of toxins (lipophilic chemical toxicants) from the body.

  • Based on recent scientific evidence, weight loss is accompanied by release of toxic, carcinogenic, neurotoxic substances that, in today’s polluted world, accumulate in our fat cells over the years.
  • Dr Ruggiero refers to a paper where the Authors demonstrated that weight loss is accompanied by increase of polychlorinated biphenyls (PCBs) in serum.
    • PCBs are carcinogenic, neurotoxic substances that also disrupt the endocrine system.
    • The Summit creators (who did not use Bravo) demonstrated that lipophilic chemicals like PCBs increased in serum during weight loss.
    • Dr. Ruggiero demonstrated through scientific references that adding Bravo increases the elimination of lipophilic chemicals that include phthalates, dioxin and many other.
    • Bravo demonstrated that it helps elimination of harmful toxicants that accumulate in fat cells over the years and are released in the bloodstream as consequence of weight loss.

Further, The most recent papers of ours clarify the mechanism of action of this formidable effect of Bravo at the genetic (DNA) level.

  • We demonstrated that in Bravo there are hundreds of different probiotic species and, unlike any other probiotic, there are phages and plasmids that confer unique properties.
  • Among these properties, we focused on the role of phages and plasmids in detoxification.
  • We feel that these results on the molecular characterization of Bravo cast a new light on the uniqueness of the product and explain its efficacy.

In particular, we are grateful to provide a safe and effective way to release weight and have the toxins managed as well.  This is also true of Lyme where toxins are liberated as the person begins to detox the condition.

In some cases, the only way to detoxify a weight loss program in today’s polluted world, is to accompany the weight loss program with an efficient detoxification program.  We know that Bravo works thanks to its unique composition.   It proves inimitable for this scope.

I am certain that you realize that the need for stress free, scientific weight loss is enormous.  You may want to convey the scientifically solid message that weight loss without detoxification can be harmful and Bravo is a valid solution.

Very truly yours,

Marco Ruggiero

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Notes from the “Toxicity and Longevity Summit”

Frustrated with traditional approaches to improving health, energy, and the immune system? Searching for ways to turn back the clock, yet nothing seems to work for you? Then don’t miss out on the Toxicity and Longevity Summit! (Full Flyer- in Link) We are lucky to have Marco Ruggier MD, Ph.D., an expert on Autism, Cancer, and the Microbiome with us to educate on not only the best diet to follow- but how to address one of the biggest roadblocks to overall health–TOXINS.

Dr. Ruggiero will present a simple, 2-phase protocol that helped Dr. Jerry Blythe regain optimal health without expensive procedures or significant side-effects. Together, Dr. Ruggiero, Dr. Stefania Paccini, and Dr. Blythe published a well-received, peer-reviewed study on the protocol and its findings.

Blythe Study Who Should Attend? **Anyone looking for cutting edge strategies to improve health and longevity. **Physicians who are looking for effective holistic solutions for their patients.

Additional Summit Speakers Peter Greenlaw,  Summit Host is a leading expert on toxins, weight loss, and longevity. Peter is the author of Why Diets are Failing Us (with Dr. Dennis Harper), TDOS Syndrome and Solutions (with Dr. Nicholas Messina), and Your Third Brain (with Dr. Marco Ruggiero) Jerry Blythe, MD, is a renowned speaker on toxins and healthy aging. A 72- year old marathon runner, Dry Blythe will share his life-changing experience from following this 2-phase protocol.

Can’t Make it in person? Register for the Broadcast and get the recording.  Hi there, You are invited to a Zoom meeting. When: Oct 26, 2019, 09:30 AM Central Time (the US and Canada) Register in advance for this meeting:https://zoom.us/meeting/register/91d9adf424085e2f7510d14dfea9e911 After registering, you will receive a confirmation email containing information about joining the meeting.

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Article from 2017

Case report: intermittent fasting and probiotic yogurt consumption are associated with reduction of serum alpha-N-acetylgalactosaminidase and increased urinary excretion of lipophilic toxicants

Jerry Blythe1 and Stefania Pacini2

1Retired Medical Doctor, Indianapolis, IN, USA 2Silver Spring Sagl, Arzo-Mendrisio, Switzerland

Corresponding author

Running title: Key words:

Stefania Pacini, MD, PhD. Silver Spring Sagl. Via Raimondo Rossi 24, Arzo-Mendrisio 6864. Switzerland.
E-mail: info@bravo-europe.com

Intermittent fasting and lipophilic toxicants

intermittent fasting; probiotic yogurt; nagalase; detoxification; thyroid

Abbreviations: N-acetyl(2-hydroxypropyl)cysteine (NAHP); 3-Phenoxybenzoic acid (3PBA), N- acetyl phenyl cysteine (NAP), Phenylglycoxylic acid (PGO), Monoethylphthalate (MEP); 2- Hydroxyisobutyric Acid (2HIB); Thyroid Stimulating Hormone (TSH); Dr. Jerry Blythe (Dr. JB); chronic lymphocytic leukemia (CLL); Institutional Review Board (IRB); Gc protein-derived Macrophage Activating Factor (GcMAF).

Abstract

In this study, we describe the changes associated with three months of intermittent fasting and probiotic yogurt consumption in a 72-year-old marathon runner with chronic lymphocytic leukemia for a number of years. Serum alpha-N-acetylgalactosaminidase (nagalase), a marker of inflammation and cancer cell proliferation, was significantly decreased at the end of a three- month observation. These results are consistent with immune modulating properties of certain probiotics based on the fermentation of milk and colostrum. Urinary excretion of non-metal toxicants that accumulate in adipose tissue such as Perchlorate, N-acetyl(2- hydroxypropyl)cysteine (NAHP), 2,4-Dichlorophenoxyacetic acid, 3-Phenoxybenzoic acid (3PBA), N-acetyl phenyl cysteine (NAP), Phenylglycoxylic acid (PGO), Monoethylphthalate (MEP) and 2- Hydroxyisobutyric Acid (2HIB) was significantly increased. These results are consistent with the weight loss (5 Kg) associated with intermittent fasting and with the known features of probiotics as detoxification tools. Consistent with certain toxicants acting as endocrine disruptors, we observed an increased elimination of toxicants and a 33% decrease of serum Thyroid Stimulating Hormone (TSH), suggesting a trend toward normalization of thyroid function. These results support the hypothesis that a combination of intermittent fasting with the consumption of specific probiotic yogurts may lead to immune modulation, detoxification and other improvements.

Introduction

A recent review of the metabolic effects of intermittent fasting published in the July 2017 Annual Review of Nutrition suggests that nutritional regimens may “offer promising nonpharmacological approaches to improving health at the population level, with multiple public health benefits”.(1) This statement is consistent with the conclusions of a study published in 2013 describing how “Intermittent fasting is reported to improve the lipid profile; to decrease inflammatory responses, reflected by changes in serum adipokine levels; and to change the expression of genes related to inflammatory response and other factors”.(2) The interest in intermittent fasting as a nutritional approach to weight loss and a number of health issues, is demonstrated by the number of peer-reviewed papers on this topic. A PubMed query for “intermittent fasting” performed in July 2017, yielded, yielded 755 results with a quasi- exponential increase in the number of papers in the past 5 years.

Intermittent fasting appears particularly efficient in achieving sustained weight loss and enhancing body composition by reducing fat mass.(3) And, a reduction of fat mass is often further associated with a release of lipophilic toxicants that may have accumulated in adipocytes(3); such a release may pose a health danger if it is not accompanied by appropriate detoxification procedures. Among the different detoxification approaches, interventions involving modulation of the microbiota appear particularly promising as they target a system, in this instance, the microbiota that is involved in all aspects of human physiology. Such interventions have received a great deal of attention in the past few years.(4,5)

Thus, it has been known for more than 10 years that probiotic microbial strains have properties that enable them to bind metals and toxins from food and water; interestingly, binding of toxicants by probiotics appears to be instantaneous, thus leading to the proposal of exploiting these features for decontamination in food and intestinal models.(6)

Based on these premises, Dr. JB, a retired Medical Doctor, embarked on a three-month experience of intermittent fasting and probiotic yogurt consumption with the goal of weight loss and detoxification. In this study, we describe the changes associated with such a nutritional approach.

Subject and methods

Dr. JB was born through Cesarean section in 1945, was fed with formulas, and suffered several early childhood infections, including pneumonia at the age of 5, treated with chloromycetin (one of the first commercial uses of chloramphenicol was chloromycetin in 1949). The drug is seldom used today because of potential toxicity and bone marrow depression. At the age of 6, he developed a passion for running that continues to this day. He kept logs of his mileage and calculates that he has run approximately 112,000 miles (180,246 Km) including a number of marathons; his remarkable achievements have recently been described in the journal, The Socionomist.(7)

He has been healthy and energetic throughout his adult life without illness other than Typhoid Fever acquired outside the United States in 1970 and treated with chloramphenicol. His bone marrow suffered a residual leukopenia and lymphocytosis which appear to have not affected his daily activities. In 2012, he was diagnosed with chronic lymphocytic leukemia (CLL) at an Indianapolis Cancer Center, and found to have a 13q14.3 deletion. Retrospective study of blood analyses, however, suggests that the condition had been evolving over a number of years and diagnosable as CLL since 2008 when for the first time he developed a leukocytosis along with his lymphocytosis. After careful consideration, Dr. JB opted for complementary/alternative approaches for his condition with particular focus on healthy nutrition and exercise, avoiding refined sugars, alcohol, carbonated drinks (sodas), cookies, pies, candy or cakes; he maintained his consistent training program that he is still implementing, running on average 6 miles per day at a comfortable pace.

In March 2017, JB embarked on an intermittent fasting program patterned on the weight loss program described by He et al.(3) Shortly thereafter, he introduced daily consumption of 100 ml of probiotic yogurt aimed at supporting the immune system and helping detoxification, choosing a product whose properties have been previously described.(8,9)

Blood and urine analyses were performed immediately before and after the three-month experience. Analysis of serum alpha-N-acetylgalactosaminidase (nagalase) was performed at the European Laboratory of Nutrients, (Bunnik, The Netherlands); the results are expressed as Units = nmol/min/mg. Blood analyses were performed at Lab Corp, Laboratory Corporation of America. Analyses of toxicants in early morning urine samples were performed at The Great

Plains Laboratory Inc. (Lenexa, KS, USA) and are expressed as μg/g creatinine. Copies of the original records are conserved at the office of Dr. JB.

Since this is a single case report that does not produce generalizable knowledge, nor is it an investigation of an FDA regulated product, Institutional Review Board (IRB) review is not required for this activity.(10) Likewise, since this case report described the experience of one of the Authors, Dr. JB, MD, the concept of “informed consent” is implicit in the authorship of the study.

Results

After three months of intermittent fasting and probiotic yogurt consumption, Dr. JB’s body weight decreased from 70.7 to 66.2 Kg (6.4%). Serum nagalase values significantly decreased from 1.92 to 1.06 U (44.8%), a value that is very close to the upper normal value set by the laboratory (0.95). Serum TSH values also significantly decreased from 7.83 to 5.2 μIU/ml (33.6%). Leucocyte count decreased from 58 to 54.9 (cells x 103/μL) (5.3%) and the percentage of lymphocytes decreased from 94 to 90% (4.4%). The number of platelets favorably increased from 93 to 125 (cells x 103/μL) (34.4%).

Concomitant with these changes, urinary excretion of a number of lipophilic toxicants significantly increased (Table 1).

______________________________________________________________________________

Table 1
Non-metal toxicants in urine______________________________________________________________________________

Compound Before After

______________________________________________________________________________

Monoethylphthalate (MEP) 2-Hydroxyisobutyric acid (2HIB) Phenylglycoxylic acid (PGO) Perchlorate

1,925 3,353 4,364 7,349 36 184 3.5 13 N.D. 0.11 70 135 0.18 1.7 N.D. 0.27

N-acetyl phenyl cysteine (NAP)
N-acetyl(2-hydroxypropyl)cysteine (NAHP)
2,4-Dichlorophenoxyacetic acid
3-Phenoxybenzoic acid (3PBA) ______________________________________________________________________________ Values are expressed as μg/g creatinine; the first term (before) refers to the value observed before intermittent fasting and probiotic yogurt consumption; the second term (after) refers to the value observed after the three-month experience.______________________________________________________________________________

It is worth noticing that the toxicants whose excretion was increased concomitantly with implementation of intermittent fasting and probiotic yogurt consumption, are among the most common environmental pollutants. It may be argued that elevated initial levels of phthalates were associated with Dr. JB’s consistent running and consumption of water from years of soft, plastic water bottles possibly containing phthalates. Interpretation of initial elevated level of 2HIB is more complex. Thus, 2HIB is formed endogenously as a product of branched-chain amino acid degradation in patients with ketoacidosis.(11) However, 2HIB is also a metabolite of gasoline additives(12), and elevated levels of 2HIB can indicate exposure to environmental pollution, exposure that could be associated with activities such as running in urban environments. Since Dr. JB had no clinical or laboratory signs of ketoacidosis, we interpret the elevated levels of 2HIB as consequence of environmental exposure.

Discussion

The changes observed during a three-month experience of intermittent fasting and probiotic yogurt consumption seem to support the hypothesis that such a nutritional approach provides health benefits ranging from weight loss to detoxification and immune system support.

Decrease of nagalase can be interpreted as the result of modulatory activities of the probiotic yogurt on the immune system. It is known that fermentation of milk leads to formation of immune-modulatory and anti-oxidant molecules, and as such, may play a role in decreasing the inflammatory status responsible for the elevated nagalase levels.(13) In addition, the probiotic yogurt used in this study contains bovine colostrum, a supplement known to have intrinsic immune-modulating properties(14) and to be rich in vitamin D-binding protein that is the precursor of Gc protein-derived Macrophage Activating Factor (GcMAF).(15) It has been proposed that nagalase is a marker of cancer cell proliferation (16) as well as of systemic inflammation.(17) Therefore, the observed decrease of serum nagalase activity, together with the decrease of lymphocytes accompanied by an increase of platelets, may reflect a positive impact of the nutritional approach described herein on the underlying pathologic condition of Dr. JB.

Such a positive impact may also be consistent with the observed decrease of serum TSH values. We interpret this change as a consequence of the increased excretion of toxicants known to act as endocrine disruptors with particular reference to thyroid function. For example, phthalates bind to thyroid hormone receptors altering the signaling of thyroid hormones (18), and perchlorate interferes with iodine uptake with resulting increased production of TSH as compensatory mechanism.(19) Thus, it can be hypothesized that increased elimination of phthalates, perchlorate and other endocrine disruptors may be responsible for the trend toward normalization of thyroid function.

The observed increased urinary excretion of lipophilic toxicants is consistent with recent report describing how such a nutritional plan followed by Dr. JB is associated with an increase of serum concentrations of lipophilic pollutants such as polychlorinated biphenyls, possibly mobilized from adipose tissue.(3) We attribute the increased urinary excretion of lipophilic

chemicals to the effects of the cleansing procedures associated with the plan described in He et al.(3) as well as to the known detoxifying effect of probiotics. For example, Lactobacillus acidophilus, a component of the probiotic yogurt used in this study, is endowed with antioxidant effects, decreasing malondialdehyde and increasing the levels of the antioxidants, glutathione reductase, superoxide dismutase, and glutathione peroxidase.(20) Other probiotic strains provide significant protection against metal toxicity by decreasing the level of toxicants in the liver and kidney, and by preventing alterations in the levels of glutathione peroxidase and superoxide dismutase.(21) Another mechanism of action responsible for the detoxifying effects attributable to probiotic yogurt consumption lays in the observation that lactic acid bacterial strains remove toxins from liquid media by physical binding(22) , and protect against toxins such as polycyclic aromatic hydrocarbons, heterocyclic aromatic amines, amino acid pyrolysates and mycotoxins.(23) In addition, it has been recently demonstrated that probiotics such as those represented in the probiotic yogurt used in this study may improve kidney function thus contributing to the overall detoxifying effect. (24)

The authors wish to emphasize that the data in this case report are from one individual, and not part of a larger study. Like Aronson who encourages reports of single cases in medicine(25) , the authors believe the history and observations may benefit those addressing the impact of environment on health.

Conclusions

The experience of Dr. JB suggests that intermittent fasting associated with probiotic yogurt consumption may lead to body weight reduction, increased excretion of toxicants and modulation of the immune system with overall positive effects on health.

Acknowledgements

The Authors wish to thank Mr. Peter Greenlaw, author of books on healthy nutrition, who introduced Dr. Blythe to the nutritional plan described in this study, and connected him with Dr. Pacini after completion of the three-month experience.

References

1. Patterson, R.E., Sears, D.D. Metabolic effects of intermittent fasting.
Annu. Rev. Nutr., Jul 17. doi: 10.1146/annurev-nutr-071816-064634. [Epub ahead of print].

2. Azevedo, F.R., Ikeoka, D. and Caramelli, B. Effects of intermittent fasting on metabolism in men. Rev. Assoc. Med. Bras. (1992). 2013; 59:167-173.
doi: 10.1016/j.ramb.2012.09.003.

3. He, F., Zuo, L., Ward, E., and Arciero, P.J. Serum polychlorinated biphenyls increase and oxidative stress decreases with a protein-pacing caloric restriction diet in obese men and women. Int. J. Environ. Res. Public Health; 2017 14: 59. doi:10.3390/ijerph14010059.

4. NIH HMP Working Group, Peterson, J., Garges, S., Giovanni, M. et al. The NIH Human Microbiome Project. Genome Res. 2009; 19:2317-2323.
doi: 10.1101/gr.096651.109.

5. Kieffer, D.A., Martin, R.J. and Adams, S.H. Impact of dietary fibers on nutrient management and detoxification organs: gut, liver, and kidneys. Adv. Nutr. 2016; 7:1111-1121. doi: 10.3945/an.116.013219.

6. Ibrahim, F., Halttunen, T., Tahvonen, R. and Salminen, S. Probiotic bacteria as potential detoxification tools: assessing their heavy metal binding isotherms.
Can. J. Microbiol. 2006; 52:877-885.

7. Blythe, J., Mailbag@. The Socionomist. March 2017, 10-11.

8. Pacini, S., Punzi, T, Morucci, G. and Ruggiero, M. Macrophages of the mucosa-associated lymphoid tissue (malt) as key elements of the immune response to vitamin d binding protein- macrophage activating factor. It. J. Anat. Embryol. 2011; 116:136.

9. Schwalb, M., Taubmann, M., Hines, S., Reinwald, H., and Ruggiero, M.
Clinical observation of a novel, complementary, immunotherapeutic approach based on ketogenic diet, chondroitin sulfate, vitamin D3, oleic acid and a fermented milk and colostrum product. Am. J. Immunol. 2016; 12:91-98. doi:10.3844/ajisp.2016.91.98.

10. John Hopkins Medicine. 102.3 Organization Policy on Single Case Reports and Case Series. http://www.hopkinsmedicine.org/institutional_review_board/guidelines_policies/organization _policies/102_3.html
Accessed August 16, 2017.

11. Konkoľová, J., Chandoga, J., Kováčik, J., et al. Severe child form of primary hyperoxaluria type 2 – a case report revealing consequence of GRHPR deficiency on metabolism. BMC Med. Genet. 2017; 18: 59. doi:10.1186/s12881-017-0421-8.

12. Benson, J.M., Tibbetts, B.M. and Barr, E.B. The uptake, distribution, metabolism, and excretion of methyl tertiary-butyl ether inhaled alone and in combination with gasoline vapor. J. Toxicol. Environ. Health A. 2003; 66:1029-1052.

13. Ebner, J., Aşçı Arslan, A., Fedorova, M., Hoffmann, R., Küçükçetin, A. and Pischetsrieder, M. Peptide profiling of bovine kefir reveals 236 unique peptides released from caseins during its production by starter culture or kefir grains.
J. Proteomics. 2015; 117:41-57. doi: 10.1016/j.jprot.2015.01.005.

14. Shing, C.M., Hunter, D.C. and Stevenson, L.M. Bovine colostrum supplementation and exercise performance: potential mechanisms. Sports Med. 2009; 39:1033-1054. doi:10.2165/11317860-000000000-00000.

15. Senda, A., Fukuda, K., Ishii, T. and Urashima, T. Changes in the bovine whey proteome during the early lactation period. Anim. Sci J. 2011; 82:698-706. doi:10.1111/j.1740-0929.2011.00886.x.

16. Thyer, L., Ward, E., Smith, R., et al. GC protein-derived macrophage-activating factor decreases α-N-acetylgalactosaminidase levels in advanced cancer patients. Oncoimmunology. 2013. 2:e25769. doi: 10.4161/onci.25769.

17. Suh, M.J., Tovchigrechko, A., Thovarai, V. et al. Quantitative differences in the urinary proteome of siblings discordant for type 1 diabetes include lysosomal enzymes.
J. Proteome Res. 2015; 14:3123-3135. doi: 10.1021/acs.jproteome.5b00052.

18. Zoeller, R.T., Environmental chemicals as thyroid hormone analogues: new studies indicate that thyroid hormone receptors are targets of industrial chemicals?
Mol. Cell. Endocrinol. 2005; 242:10-15.

19. Leung, A.M., Pearce, E.N. and Braverman, L.E. Perchlorate, iodine and the thyroid. Best Pract. Res. Clin. Endocrinol. Metab. 2010; 24:133-141. doi:10.1016/j.beem.2009.08.009.

20. Bagherpour Shamloo, H., Golkari, S., Faghfoori, Z. et al. Lactobacillus Casei decreases organophosphorus pesticide diazinon cytotoxicity in human HUVEC cell line.
Adv. Pharm. Bull. 2016; 6: 201–210. doi:10.15171/apb.2016.028.

21. Majlesi, M., Shekarforoush, S.S., Ghaisari, H.R., Nazifi, S., Sajedianfard, J. and Eskandari, M.H. Effect of probiotic Bacillus coagulans and Lactobacillus plantarum on alleviation of mercury toxicity in rat. Probiotics Antimicrob. Proteins. 2017; Jan 13. doi:10.1007/s12602-016- 9250-x.

22. Haskard, C.A., El Nezami, H. S., Kankaanpää, P. E., Salminen, S. and Ahokas, J.T.
Surface binding of Aflatoxin B1 by lactic acid bacteria. Appl. Environ. Microbiol. 2001; 67:3086– 3091. doi: 10.1128/AEM.67.7.3086–3091.2001.

23. Zoghi, A., Khosravi-Darani, K. and Sohrabvandi, S. Surface binding of toxins and heavy metals by probiotics. Mini Reviews in Med. Chem. 2014; 14:84-98.

24. Abbasi, B., Ghiasvand, R. and Mirlohi, M. Kidney Function Improvement by Soy Milk Containing Lactobacillus plantarum A7 in Type 2 Diabetic Patients With Nephropathy: a Double- Blinded Randomized Controlled Trial. Iran J. Kidney Dis. 2017; 11:36-43.

25. Aronson, J. K. Unity from diversity: the evidential use of anecdotal reports of adverse drug reactions and interactions. J. Eval. Clin. Pract. 2005; 11:195–208.

Authors’ Contributions

Jerry Blythe, MD: performed the experience described in this study, provided critical input and assisted in revising and improving the paper.

Stefania Pacini, MD, PhD: wrote the first draft of this paper, provided critical input and assisted in revising and improving the paper.

Disclosures

Jerry Blythe discloses no conflict of interest. He bought all the foods and supplements used during his experience and paid for the analyses reported in this study.

Stefania Pacini works as external consultant for Silver Spring Sagl, the company producing the probiotic yogurt used in this experience. She had no prior knowledge of the nutritional plan followed by Dr. Blythe nor of the results of the analyses that were communicated only after completion of the experience.

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