Iodine patch test

https://www.globalhealingcenter.com/natural-health/how-to-perform-the-iodine-patch-test/

 

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This product gives your body things it needs to convert D3 into GcMAF

A new product by Mimi’s Kitchen Club!

GcMAF Support – 100 capsules – a three months supply.

This product gives your body things it needs to convert D3 into GcMAF

If you are eating wheat, grains, or  GMO’d Roundup ready foods. If you are exposed  glyphosate/Roundup.   It converts into glyoxylate.  Oxalates share the same transport system as sulfation.  This causes poor sulfation of D3 where it cannot sulfate D3 into GcMAF.   The following nutrients are needed to sulfate D3.

  • Zinc
  • DAO
  • Lysine
  • P5P
  • B6

Sterling Hill describes it on the mthfrsupport.com website:  Thanks to Earl for sharing this.

https://mthfrsupport.com/2016/05/what-sterling-thinks-are-the-most-important-genes-on-the-variant-report-to-date/

What can I do to support the Vitamin D Receptor incase a gene is impaired?

VDR: When the vitamin D receptor gene is impaired, this can lead to many health problems. Vitamin D hormone is needed to break down into GcMAF which is then needed to attack stealth pathogens, viruses and cancer cells.

Did you know that you can only get sulfated D from the sun between 8am and noon?
Many chronically ill do not get out of the bed to get sulfated D from the sun. Also many chronically ill people are low on GcMAF because they are low on D3 which needs to be sulfated and and broken down into GcMAF.

Ever wondered why most people with cancer are D deficient? Because GcMAF which is one billionth of a gram in the blood is crucial. GcMAF has six attacks on cancer including the cannabinoid pathway. That is why many who do CBD oils have shrunk their tumors. (imuno does the same)
We also see that many people with viral overloads, autism, cancer and lyme are GcMAF deficient. They may even have D levels close to 100 but they are not sulfating their D3 because of the following:

Eating GMO’d Roundup ready foods. Glyphosate converts into glyoxylate. Oxalates share the same transport system as sulfation. This causes poor sulfation of D3 where it cannot sulfate D3 into GcMAF.

  • Zinc is needed to sulfate D3
  • DAO is needed to sulfate D3
  • Lysine is needed to sulfate D3
  • P5P is needed to sulfate D3
  • B6 is needed to sulfate D3

Get these valuable precursors in the new product from Mimi’s Kitchen Club to support the process of sulfating Vitamin D3 into GcMAF.

New Mimi’s Kitchen Club Product:

“GcMAF Support”

GcMAF Support – 100 capsules – a three months supply.

Precursor Support to Sulfate VITAMIN D3 to GcMAF

Ingredients in this product:

  • Zinc Gluconate is important for low-excretory nerve function, wound healing, protein synthesis, and it supports immune function. (2)
  • DAO, or Diamine Oxidase is a digestive enzyme which breaks down excess histamine, helps regulate specific functions of your digestive, nervous, and immune systems. (3)
  • L-Lysine an essential amino acid, helps curb virus, reduce anxiety, promotes wound healing, builds muscle and prevents bone loss.  Without it, we risk insufficient hormones and immune cells. (1)
  • P5P is a coenzyme which is the active night form of Vitamin B6.  It regulates metabolism at the cellular level, muscle growth and repair, mood regulation, sleep regulation, and other executive functions by assisting the enzyme action. (6)
  • B6 Pyridoxine is a water-soluble vitamin needed for protein, fat and carbohydrate metabolism and the creation of red blood cells and neurotransmitters. (5)

A few things Mimi added in small amounts to round out the formula

  • Bao He Wan is popular Chinese Herbal preparation for indigestion, bloating, reflux, nausea, diarrhea and food stagnation. (4)
  • PQQ (Pyrroloquinoline Quinone) – is a coenzyme, like CoQ10.  CoQ10 optimizes mitochondrial function, PQQ activates genes that govern mitochondrial reproduction, protection, and repair. (7)

It doesn’t hurt to have what you need!

GcMAF Support – 100 capsules – a three months supply.

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The Holistic Doctor Deaths

Recap on my Unintended Series: The Holistic Doctor Deaths…

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When I broke the news to the health world, as gently as I could, about the beloved Jeffrey Bradstreet  MD, I never intended it to become some kind of series.

I was honored to do the first interview with the Bradstreet family, and commend them for their bravery as they search for the answers.

My better half (a holistic doctor) and I knew several of the doctors well, and our hearts go out to their friends and family. I cannot even imagine what they’re going through.

I appreciate the few media organizations who have had the guts to share these stories; including the New York Times, (who wrote me requesting info), The Guardian UK, and  network affiliates around the US. Sadly, most mainstream sites barely cover one doctor, let alone the dozens we’ve had die this year if we count mysterious accidents, murders, alleged suicides or unexplained sudden deaths.

As you will see in the timeline below, there are allegedly 3 different dates within a month where  2 doctors died on the same day. That’s 6 doctors  (who died in pairs on 3 different days) which is almost half out of the 13 doctors in the official series. 

Some of the biggest skeptics, who rolled their eyes at the first few deaths, are now wondering if there isn’t a connection.  There have been theories, from GcMAF to CBD oil, but I don’t think all doctors used both of these treatments. I’m not convinced either is the smoking gun, but might hold the answer.

Obviously we have several unsolved murders here (and some alleged suicides, most still under investigation), and I sincerely hope they get to the bottom of this as again, we knew several of these amazing doctors personally.

Besides the fact that the majority were holistic, there is another common theme; they all cared deeply and were passionate about health. I have endless emails from their friends, patients, neighbors, loved ones, and family members. It’s clear they were adored by many.

I hope they are never forgotten and that others will carry on their work and not live in fear.

These healers, some of whom were best selling authors,  were  kind, caring people who have left this world a better place; I’m honored to have known them, and am still studying their work today.

Sign up for the newsletter for more information and stay safe and strong. While it’s always good to be aware, I’ve learned that living in fear doesn’t help us at all.  

See below for the official detailed timeline

1) June 19th, 2015 – Dr. Bradstreet formerly of Florida, and now practicing in Georgia, was found with a gunshot wound to his chest in a river. The small town locals ruled the death almost immediately as a suicide, but many have their doubts. This same day in Mexico, June 19th, 2015, three doctors were traveling to the State Capital to deliver some papers and were reported missing. This is the only case outside of the U.S.. Authorities said they found the bodies, but the families say those bodies look nothing like their family members and they are demanding more proof and more testing. A sad but riveting article was written about those details here. I also did an exclusive interview with the Bradstreet family as they publicly said I was the only journalist they trusted to talk about the horrific incident.

2) June 21st, 2015 Father’s Day, Florida East Coast: Two chiropractors are found dead. Their names are Dr. Baron Holt and Dr.Bruce Hendendal, both fathers. Dr. Hedendal also held a PhD in nutrition from Harvard. Both were presumably healthy and described as very fit. There is still no cause of death listed, for either doctor  in the articles we find. A few people have contacted me about Dr. Hedendal, 67, but admit that they were surprised by his death and still find it shocking. (Update: Family and loved ones of Dr. Hedendal have spoken to the media that they are suspicious of his death and want answers. Interestingly, Dr. Holt (33), lived in North Carolina which is the state where Dr. Bradstreet’s body (the first doctor to be found) was discovered two days prior. Dr. Holt was visiting Jacksonville, Florida, though, when he died there. Dr. Bradstreet (see story #1) was living in Georgia at the time of his death and before that he lived in the neighboring state of Florida. A few mutual friends of Dr. Holt have contacted me. They are doctors and have shared with me a cause of death that I find rather shocking and cannot confirm, therefore, out of respect for the family, I will not list that here. Both were well loved by their patients, community, friends and family.

3) June 29th, 2015 – The beloved holistic Theresa Sievers, MD was found murdered  in her home. Her co-worker says she was known as the “Mother Teresa of South Florida.” Her husband and children were in Connecticut at a family reunion when she was murdered. The authorities have been investigating for two weeks “around the clock,” and now say that she was targeted. Her murder was not random nor was it a home invasion and when the facts come out “…books and movies will be written about it.”, it’s that big of a story.  On the very same day, June 29th, Jeffrey Whiteside, MD, a pulmonologist, went missing- vanishing when he simply “walked away”. Dr. Whiteside, known for his successful treatment of lung cancer, disappeared in Door County, Wisconsin while vacationing with family. He was on foot and numerous reports have called it “mysterious,” saying he vanished without a trace. They’ve been searching now for three weeks and even colleagues have joined in (along with bloodhounds, drones search parties, and helicopters) but not one shred of evidence has surfaced. (UPDATE: Two arrests were made in the death of Teresa Sievers and one of the assailants called himself the “Hammer” on Facebook (Dr. Sievers was allegedly killed with a hammer). The other was one of her husband’s childhood friends who was just recently extradited to Florida. His attorney says there is evidence to show he had no involvement whatsoever with the crime. Her husband, Mark Sievers, has not been arrested at this time.

4) July 3, 2015 – Patrick Fitzpatrick, MD goes missing. He was traveling from North Dakota to neighboring Montana (which he did often as his son lives there) and his truck and trailer were found on the side of the road. The search has expanded but authorities say it’s as if he vanished without a trace. He’s 6′ tall and described as Irish-looking with a goatee (details can be found in the links).

5) July 10th, 2015 – Lisa Riley, 34 years old, DO (Doctor of Osteopathic medicine) is found in her home with a gunshot wound to her head. Her husband who found her and called 911, has a prior record. He was previously charged with the attempted murder of his ex, Ms. King, before charges were eventually dropped. Evidence showed that there was gun residue found on Ms. King’s hand and not Mr. Riley’s. Riley’s story corroborated this, but King’s allegedly didn’t, and her story kept changing (click link in this paragraph for their story with details on all of this). Mr. Riley has since been charged with the death of his wife, Lisa Riley. We’ll see what happens in court.

6) July 19, 2015  – A month to the day after the first doctor (Jeff Bradstreet, MD) was found dead with a gunshot wound to his chest, Dr. Ron Schwartz was found murdered in his E. Coast Florida home. Sadly, he was shot to death. We don’t know if he was holistic, but he was licensed and lived between Florida and Georgia. He was a gynecologist who lived in an unincorporated part of Jupiter, Florida on a few acres of land and there are reports he ran an organic lawn service on the side.

7) July 21, 2015 – Dr. Nicholas Gonzalez, a holistic  MD, dies suddenly. The same day another holistic doctor is also found dead (see below) . Both of alleged heart attacks, though their own friends and family say publicly it doesn’t add up.  I had met Dr Gonzalez and his amazing wife, and my better half has interviewed him several times.  His official website says that while they initially thought it was a heart attack, his autopsy does not support that belief and shows no heart attack. They are awaiting further tests. “Dr. Gonzalez was in excellent health otherwise so his passing is quite unexpected.” It is reported that he was alone at the time of his death. Countless people every week (including close friends) write me questioning the fact that he died “naturally”. He also said in several interviews (including the last one before he died) that he thought big pharma wanted him to get hit by a bus or that he might die suddenly.  Frightening metaphors and a mystery we hope to get to the bottom of soon.

8) July 21st, 2015 – Dr. Abdul Karim, an outspoken and loved holistic (biological) dentist, 41 years of age, is found dead alongside the road by a passerbyHe had been preparing for a half marathon and was allegedly out training that night. His old fraternity brother (lifelong friends), friends of many years, and patients said it didn’t add up. He was in perfect shape and ate an extremely healthy diet. Mainstream news said he had a “massive heart attack” but many who knew him have written to me with their sincere doubts.

9) July 23, 2015-  Authorities announce they have found the body of Dr. Jeffrey Whiteside who has been missing for over 3 weeks. Chief Deputy Pat McCarty said at the news conference on Thursday that a .22 caliber gun was recovered at the scene. Investigators believe the body was there for “some time”.

10) August 12, 2015  – Osteopath Mary Bovier is found slain to death in her home in Pennsylvania. Her significant other, who we are told is also an osteopath, was questioned and released. At this time there have been no arrests and there are no suspects.

11) August 18, 2015 – I report that Jeffrey Whiteside’s death is officially ruled as a suicide. But the local press calls the investigation a “mess” (described here in detail). Many are shocked at how long it took for details to emerge and countless people write me with their doubts. Even local authorities were asked by the local press if they’d heard of my stories. They admitted they had, but hadn’t been contacted by the feds on the case.

12) September 7, 2015 – I broke the news to the health community of 29 holistic doctors (also referred to in the German press as homeopaths/ND’s/psychiatrists) who were found poisoned by an overdose of a hallucinogenic drug. I’ve done several updates of the articles, using a German translator, but the whole story of what really happened has still not been revealed. One thing most can agree on; it was a large overdose and mainstream German news reports that some were in “life threatening situations” when dozens of ambulances and a helicopter arrived to the chaotic scene. The good news? All lived and have allegedly made a full recovery (though some had to be restrained for their safety for some time).

13) September 16, 2015 – I reported that our colleague, best selling author and holistic MD Mitch Gaynor, is found dead outside his country home; an hour or so from Manhattan where he practiced. We knew Dr. Gaynor and had just finished an advanced copy of his book that he had sent to us. It was quickly called a suicide by the local sheriff. The NY Times wrote me asking for details and we had a nice exchange. Even though I have gently broken the news on this and many other stories, the NY Times fails to source me (I didn’t expect them to source HealthNutNews, though the Guardian UK and other mainstream sources have for some time). UPDATE: Mitch Gaynor MD had been on RT not long before he died. We wanted to contact them for comment, but soon after Mitch was found dead? the founder or RT was found dead in his Washington DC hotel room.

14) Sunday October 11th – We announce that Dr. Marie Paas is found dead of an alleged suicideShe was well known for her animal advocacy.  People on her Facebook page seemed shocked and in disbelief about her death. Unlike the 3 others by this date who were alleged suicides (one who has been officially confirmed) we don’t know the location where Dr. Paas was found, nor the details of her alleged suicide. Many seem concerned for her pets, who they say she loved very much

15) October 29th 2015, Jerome E. Block, M.D,  jumped to his death from Central Park West residence.  Authorities say Block jumped from the 20th floor of his residence around 9:30 a.m.  Details and video can be found here.

16) 2015 dozen plus accidental deaths and freak accidents, mostly holistic doctors; Besides the 13 doctors covered thus far who died in non accidental deaths, we have 5 chiropractor deaths  that were accidents. We also have more deaths of doctors here:  some described as freak accidents.  Again, we didn’t include them in the official unintended series.  We  have the famous holistic ABC News doctor, Jamie Zimmerman,  who allegedly slipped and fell and hit her head in Hawaii while on vacation, and who was carried out to sea.  We also have more accidental deaths of holistic doctors here in NY (accidental), and yet  another here . We literally couldn’t keep up at times there were so many. And yet another holistic MD found dead which I wrote about here. It doesn’t stop.

17) Late December 2015 – January 2016 I didn’t include PhD’s in the series (unless they were also an MD, DO or DC) but then just this past few weeks we saw 2 Canadian PhD’s/researchers killed. One was stabbed in what his friends called a safe neighborhood Another, a Phd (who was a wife and mother of two)  was hit while jogging near the beach and then buried in a shallow grave on the tourist  island of Grenada. Then days later a holistic doctor is also attacked there with his wife (physician’s assistant)  in this same tiny island of Grenada. (see #18) This is where we suddenly start seeing a spate of cancer researchers killed which hadn’t happened previously and then suddenly we have a one every few days.

18) January 23, 2016, A top MD, John Marshall, 49,  who was a former 4th generation marine, had served in Afghanistan, and had survival training, was found dead in the Spokane river and they pulled his body out that day. He was said to be  in great shape, was doing his usual run in Seattle near a river. Authorities say he somehow must have fallen into the river and drowned and almost immediately said it was death by drowning. UPDATE! Dr Marshall’s wife (also a doctor and surgeon) has hired her own investigator and is questioning the quick call by Seattle PD. Again, more families are questioning these deaths and what some find absurd rulings by authorities.

20) January 25th, 2016  Our own friend of ten long years, holistic doctor Rod Floyd, was found dead at his home here in Florida. He lived a matter of miles from us and I’d been to their home as they’d been to mine. It was definitely not suicide in any way and his wife of 37 years says he was “hearty and healthy” and calls it an accident. That’s all I’ll elaborate right now, as even I don’t know all the details. Dr Floyd did buy a sauna from me (but that wasn’t involved in his death which I was told initially) Besides being a chiropractor, Dr Floyd was also a clinical professor at Palmer Chiropractor College near where we live. He and his wife also had run the Natural Awakenings magazine for some time.  Our whole community is shocked and saddened by his death.

21) January 26, 2016. Believe it or not just a few short weeks later, a holistic MD and his wife (a physician’s assistant) were attacked, while on a relaxing beach stroll, by a sword wielding man in the same place- the tiny island of Grenada. The holistic MD survived. Sadly his wife was murdered and raped by the machete wielding attacker. Both perps in each attack of the doctors in Grenada in a couple of weeks are reported to have  been  recently released from prison and in both crimes (allegedly unrelated)  the men just nonchalantly turned themselves in after the crime. Some experts have their theories on this. I won’t elaborate at this time. Grenada locals are shocked and say they’ve never seen anything like this- let alone twice in a row.

22) January 28th, 2016  The new spate of cancer researcher/doctors  who are killer or found dead doesn’t slow down and this might be the strangest of them all:  Yet another cancer researcher, Professor Alan Clarke, 52, was found dead in the wood in hanging from a tree in a rubber body suit on December 28th, 2015 and they ruled it accidental.

23) February 1, 2016  Just a week later, to the day, after losing our local friend and holistic doctor; Rod Floyd we lost another local friend also right here in our same local holistic community. The wonderful young Paige Adams FNP, B-C, also lived just up the road here locally in Florida was found dead at her home. She had worked closely for many years with her good friend and colleague Dr. Bradstreet. The problem is Dr. Bradstreet started off this whole holistic series when he was found dead in a river. Now we have his close friend (and our friend!) and his colleague of many years  found dead.  She was so young so this is particularly heartbreaking. Paige Adams had asked to stop by the house to see us days before she died. I feel awfully for not taking her up on that, and my heart goes out to her family and patients. She is the only one in this entire series I’ve ever written about who is not a doctor of some sort (DC, MD, DO, and even a PhD at the end) But she was a board certified Nurse Practitioner whose patients rave about her endlessly. She also stated publicly (which you’ll see in the link) that if anything happened to her don’t assume it was an accident.

23) February 14, Valentine’s Day. Cancer HIV Researcher in Seattle- Cheryl Deboer is found dead in a culvert with a plastic bag over her head. Investigators oddly quickly they can’t see any evidence it’s a homicide, but her mother and other relatives state publicly  there is no way she crawled 1.5 miles through brambles and water and mud to put a plastic bag over her head in a culvert. They stated that if she would have wanted to take her life (and listed plenty of reasons why she wouldn’t with a happy family and job at the famous cancer research lab) she could have done it much more easily. The general public tends to agree with the family. Families are now starting to question these ridiculous statements called so quickly by local authorities. Some are hiring their own investigators and doubting local authorities. Many are wondering if the local authorities are to be trusted.

24) March 10, 2016 Yet another cancer researcher found dead in a field. He was only 34, and found in a safe quaint area in the countryside and people are astounded. Nearly immediately authorities announce his death is ‘not suspicious’, but there is a public outcry of how that could be so. We still have zero answers as to his cause of death.

25) March 23, 2016 Wednesday, Triple homicide: Prominent Holistic doctor Henry Han, was found murdered in his 7 acre Santa Barbara Estate along with his wife and daughter, only 5 years of age. All were shot and murdered and wrapped in plastic. Quickly an arrested is made of a young man who has no priors who lived with his father oceanside in Santa Barbara. His father was EX CIA. We will update as details emerge.

26) April 6, 2015   Dr Harsch, 59, a self described holistic medicine MD was killed while riding his bicycle with a group of friends. He was the only one hit and none of the others were injured whatsoever. See details here. (Editor’s note: We had another holistic doctor earlier in the series also killed on a bicycle ride last year and many of her friends and loved ones contacted me about their concerns and questions on her death.

I will continue to report on these stories despite death threats, being put on a hit list that was posted publicly (which we think is fake- but who makes up that kind of stuff?).  I’ll be speaking at a few events in 2016 on the subject, one will be a group of doctors with several thousand in attendance.

I don’t want other doctors to live in fear. I know many have shut down their practice or hired bodyguards due to these reports.  I understand it’s necessary to be aware and safe, but I hope that those brave enough to work in holistic medicine will keep carrying the torch and doing their great work, in memory of those we lost this year.

Sincerely,

Erin Elizabeth

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Q: Is there a difference between Bravo Yogurt and imuno?  

Q: Is there a difference between Bravo Yogurt and imuno?                                                              (I am happy with my yogurt.  Do I need imuno?)

❤️🌟🙏🏻

A:  Bravo Yogurt and imuno are built to work together and can be used separately but they are not the same.  Both of them lift and turn on the immune system.  They do so in separate ways. They are both restoratives!  In my experience, imuno works faster to remove disease from a cellular environment and lifts the innate immune system.  Bravo builds a factory supplying many functional needs of the body.  Neither one can be ignored since they are needed.

Bravo GcMAF Probiotic Yogurt “sets up” its factory by attaching to the mucosa lining.  This is the gut lining in the esophagus, stomach, duodenum, small and large intestine and lymph.  It’s “factory” reads our body specifically and replenishes the chemistry components as needed to balance your body needs in a customized way for each individual.  More specifically, it has been specifically designed by to create GcMAF which makes it different from any other probiotic yogurt or drink!  GcMAF is the molecule that turns on the immune system.  Thanks to Dr. Ruggiero who studied the yogurt, microbiota, and the immune system, Bravo Yogurt is designed it to supplement the natural system of immunity ignition that takes place in the Vitamin D function and our vital defenses.

Those who have low Vitamin D, feel gloomy and powerless most likely have viruses which have accumulated in the body.  These viruses have a world of their own and when they get activated and fed by a constant stream of carbs and sugars, they begin to nest and reproduce more rapidly.  They use the biofilm from their nest to glue down the doors that the GcMAF would naturally come out of leaving us with a feeling of helplessness and “I can’t win.”  This turns into lack of confidence!  Breaking that cycle is what we do!

One key factor in breaking the cycle of viruses and sugars is to go on a low carb no sugar diet.  The doctor recommends, the Ketogenic Diet which provides plant-based food which has the DNA of the plant’s immune system and protein for strength. This food does not create micro-inflammation which clogs the drainage process. This diet gives us a leg up on getting the easily completing digestion so that we can move on to detoxifying disease.

Also, easily digestible plant food s and healthy oils helps us by saving resources!  You could imagine if each day someone came into your home and lit off a bomb of goo that exploded all over your house!  It would make you anxious and overwhelmed.  Yet do you know that you even do that?    Being mindful and making less clean up allows us to move a forward productive direction.  Do you even know that you are blocking your forward movement?  The gut gets slippery and we loose our “grip” for forward movement.   It is easily fixable and even saves you money.  But it is work because you will start making progress digesting your emotions.

Digestion takes energy!  Eat only what your body needs!!  Don’t eat to push down your emotions.  Digest everything before you go to sleep and your body will rumble and grumble as it digests the emotions and thoughts of the day.  This will reduce the clean up in so many ways.  Add some clean healthy, filtered or spring water to bring a little gurgling brook to clean your system.  Remember!  Undigested food has access to go straight to your brain and create fatigue.  Don’t want that?  Simple awareness is often curative!

Use your freed energy to heal. Bravo Yogurt costs about $30 or less per month.  It makes customized nutrition, it will lead you to eat better if you pay attention, it created GcMAF and begins to turn on your immune system in weeks.  You will feel lighter, brighter, more confident, forward moving, and the gears start to work together.  I think of it as a good food.  It is like fasting in that it takes the toxins from deep inside the layers of tissues and it turns on essential functions in a cascade but that isn’t all!  When the immune system turns on, I begin to feel more aware, more confident, younger, more efficient, more productive, and more connected with myself.  I am more connected to WHO I AM in an essential way.

imuno

imuno® The Solution cleans and restores. It takes its chondroitin sulfate and adheres itself to the cell walls.  This is the collective brain of the body waking up the brain power and cellular power. The phospholipids act as a mild but very sparkly natural “soap” and once clean, your cells have better visibility and awareness and can find it’s comfort in being our best self back again! Then the penetrating rays of the sun through the form of Vitamin D moves into the power centers of the cells and turns them on.

The imuno works in a small specific area at first, about the size of a softball. By continued application doses of a few drops 2 times per week, it can keep that area clean and move the actualized area out to a greater size.   Take care not to take too much or the body will need more power to keep you lively while the imuno’s process is moving out everything these awakened cells don’t need.  Don’t be surprised if your outer life-based behavior begins to change and becomes more efficient and you enjoy cleaning more spontaneously.  “Easy does it”. You are creating an excellent flow that you have the resources to manage.

Both the Bravo GcMAF and the imuno keep the tissue more alive both within the cell and in the fluids around the cells.  They remove pollution and growth of unwanted microbial activity and remove long standing celluar communities of bad microbes.  The Bravo Yogurt keeps the motion fluid and shimmers the tissue keeping it alive and wholesome.  The two together are like having the movers and cleaners come and clean up the inner environment. Life changes!!  You become more efficient.  Hopefully, you will get the things out of your way which weigh you down!   Like maintenance everywhere, it will need to be done regularly.  The results of this effort are comfort and healthy.  They leave you to move forward, be more efficient at what you know you can do, and to enjoy life.

How to use imuno

There are several applications for using imuno.  It can be applied sublingually (drops under the tongue), by a topical salve (that works as an injection), enteric-coated capsules to activate the solar plexus area, or suppositories to activate the pelvic area.  It can also be used under medical care as an injection into an issue or tumor site or used as an IV.    In my experience, suppositories are especially needed for long term use an can give access to an opening at the back of the sacrum that opens into the spinal area.  Cleaning the area inside the spine removes heavy metals, bacteria, and viruses that have been growing there for a long time.  Using suppositories in this way is like cleaning a soapy bottle until it removes the diseases.

Lastly, the Vit D penetrates into the cells, turning on its mitochondria and powerful systems that were asleep due to lack of ignition and fuel.  You will feel great changes in 4 months of doing imuno as the literature now shows us.  imuno® also cleans out everything around the cells too. It makes an amazing clarity and grace where everything is working and easier.  Externally, you might experience less struggle and more strength  to get the job done.

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GUIDE TO AND RETROVIRUSES

Jay-Davidson-Ultimate-Supplement-and-Detox-eGuide-to-Viruses-and-Retroviruses

VIRUSES

ULTIMATE SUPPLEMENT AND DETOX GUIDE TO

AND RETROVIRUSESBY: DR. JAY DAVIDSON

WEBSITE:
AT-HOME PROGRAM:
ULTIMATE COFFEE ENEMA PROGRAM: MICROBE FORMULAS:

www.DrJayDavidson.com www.AtHomeProgram.comwww.UltimateCoffeeEnema.comwww.MicrobeFormulas.com

RETROVIRUSES AT A GLANCE

Retroviruses are responsible for many deadly diseases including cancer, leukemia, and AIDS. Retroviruses, unlike typical viruses, are RNA viruses.
An enzyme in retroviruses transcribes their RNA into a DNA molecule that can integrate with a host’s DNA. Once in a host cell, a retrovirus can take control of the cell’s machinery and replicate itself. Retroviruses are passed down generationally. Mothers can pass them to infants. Retrovirus DNA embedded in the DNA of human ancestors accounts for up to 8-15% of the DNA in the human genome.

HERVs (human endogenous retroviruses) are passed down from ancestors. HERVs can become active and start replicating, causing or exacerbating illness. Generally, HERVs can cause immune deficiency.

Retroviral pathogens release biotoxins into their hosts, leading to toxic overload. Retroviruses can jump the species barrier from non-human primates to humans. Retroviruses could play a role in addictive behavior. Retroviruses have been found to create mutations in human DNA.

The cell danger response (CDR) is an evolutionary protection mechanism. Retroviruses can trigger this response, leading to many adverse effects.There is an unexplainable amount of retrovirus in some, if not all, vaccines. Reactivation of HERVs is a serious concern. It can lead to system-wide dysregulation in the body No laboratory test can identify retroviral infection. Immune markers are used for indirect diagnosis.

Plants, like animals, have evolved in the presence of retroviruses and have developed protective mechanisms against them. Herbal and nutritionalsupplements are very effective against retroviral infection. Cistus incanus is a natural antiviral, antibacterial, and biofilm buster. Extracts of broccoli sproutscontain antiviral compounds.

Active ingredients in herbal remedies St. John’s Wort and Chinese Skullcap have antiviral action. Adding a high-dose selenium supplement is important to any retroviral elimination protocol. Bioactive carbon supplements have toxin-binding capabilities far beyond activated charcoal.

Other herbs and supplements that facilitate and support an antiviral protocol include:

Green Tea

Lomatium Root (Lomatium dissectum)

LOMATIUM ROOT

Pantethine (B5)

B5

Stevia

Stinging Nettle (Urtica dioica)

Luteolin

LUTEOLIN

Vitamins D3 and K2

3
2

Olive Leaf

Binders

BINDERS

Reishi Mushroom

Bitter Melon

Bioactive Carbon

BIOACTIVE CARBON

Bioactive carbon supplements are systemic toxin binders. They are not sourced from activated charcoal. Activated charcoal, a long-chain, or “spent” carbon, binds toxins in the GI tract only.Bioactive carbons are specialized to enable increased binding ability. Many contain high-energy long, medium, and short-chain active carbon molecules. A variety of chain lengths allows thecarbon supplements to bind to different things in different areas of the body.8

In addition to their superior biotoxin binding abilities, bioactive carbon products support cellular respiration (cellular energy production). These products provide the carbon, as well as hydrogen, and oxygen molecules that comprise the body and the cells. Therefore,this organic material repairs the body.

3 Rebensburg, Stephanie, et al. “Potent in Vitro Antiviral Activity of Cistus Incanus Extract against HIV and Filoviruses Targets Viral Envelope Proteins.” Scientific Reports, Vol. 6, No. 1, Feb 2016. Web

4 Furuya, et al. “Sulforaphane Inhibits HIV Infection of Macrophages Through Nrf2.” PLoS Pathogens, Vol. 12, No. 4, 19 April 2016. Web

5 Jacobson, J M et al. “Pharmacokinetics, Safety, and Antiviral effects of Hypericin, a Derivative of St. John’s Wort Plant, in Patients with Chronic Hepatitis C Virus Infection.” Antimicrobial Agents and Chemotherapy, Vol. 45, No. 2, Feb 2001. Web

6 Li, BQ et al. “Inhibition of HIV Infection by Baicalin–a Flavonoid Compound Purified from Chinese Herbal Medicine.” Cell Mol Biol Res, Vo. 39, No. 2, 1993. Web

7 Steinbrenner, H et al. “Dietary Selenium in Adjuvant Therapy of Viral and Bacterial Infections” Advances in Nutrition, Vol. 6,No.1, Jan. 2015. Web

8 www.microbeformulas.com

EBV

WHATYOU NEED

TO KNOW

Most people become infected with EBV as infants or young children. And the shocking fact is that 95% of people are infected at some time in their lives.9 It spreads remarkably easily viasaliva or other body fluids. Although many people carry of this virus without apparent effects, some will experience moderate to debilitating symptoms that can make life challenging.10

EBV is part of the herpesvirus family and is also known as human herpesvirus 4. That’s the same viral family that causes herpes, shingles, and chickenpox. Fortunately, if you get EBV as an infant or young child, you typically don’t get very sick and probably won’t remember experiencing an infection. Mononucleosis, the kissing disease, is the manifestation of EBV that most people recognize.

If you miss out on it as a child, you can get exposed to it later in life. If you happen to have a compromised immune system that cannot fend off the virus at the time of exposure, you could develop infectious mononucleosis (IM).

9 “Epstein-Barr: Scientists Decode Secrets of a Very Common Virus That Can Cause Cancer.” ScienceDaily, ScienceDaily, 15 Dec. 2010. Web

10 Cohen, Jeffrey I. “Optimal Treatment for Chronic Active Epstein-Barr Virus Disease.” Pediatric Transplantation, U.S. Nationa Library of Medicine, June 2009. Web

INFECTIOUS

MONONUCLEOSIS

IM is typically more severe than the infection that happens in childhood. Signs and symptomsof IM may include:

SYMPTOMS OF CAEBV INCLUDE:

Severe fatigue

Swollen lymph nodes in the armpits and neck

EBV Reactivated

Headache

Fever

Swollen tonsils

Skin rash

Soft, swollen spleen

Extreme fatigue

Sore throat

Swollen lymph nodes (lymphadenopathy)

Swollen liver and liver dysfunction

Low numbers of platelets (thrombocytopenia)

Rash

Emotional disturbances and stressors

Autoimmune diseases, like Hashimoto’s thyroid

Fever

Irritation of mucous membranes

Enlarged spleen (splenomegaly)

Anemia

Increased susceptibility to other infections

Chronic achiness

A variety of debilitating neurological symptoms

A variety of debilitating neurological symptoms

Sore throat that doesn’t get better after treatment with antibiotics

When something triggers reactivation of dormantEBV, it can come roaring back with a vengeance. Chronic active Epstein-Barr virus (CAEBV) can be like having extreme mononucleosis or severe chronicfatigue syndrome. Symptoms can come and go for years. Severe cases may include evidence of liver dysfunction, immune suppression,
and anemia.

SO WHAT CAN I DO FOR VIRUSES?

Chronic EBV and retroviruses don’t respond to the typical nucleoside antivirals, and scientists have demonstrated the reason: antiviral agents work by blocking DNA polymerase, which inhibits viral replication inside cells. Chronic EBV infectiondoes not need DNA polymerase to replicate itself.11 Therefore, current antiviralagents have no effect on chronic EBV infection. There are no medicines to get rid of EBV, nor are there vaccines to help prevent it.12

Conventional medical treatments primarily focus on addressing the symptomsassociated with EBV infection and little more. However, there are natural alternatives that not only target EBV but also work to support a compromisedimmune system that may be allowing the virus to run amok.

An updated therapeutic approach to health and wellness should include EMR/EMFcontrol and protection and other vital steps to keep the environment of our bodiesnhospitable to pathogens. It involves addressing toxic overload from Lyme disease and coinfections, parasites, heavy metals, and mold. Elimination of environmental toxins and biotoxins is crucial.

Since oxidation is how EBV drives its progress, antioxidants are a significant defense.13 Retroviral and viral infections are not to be taken lightly. They are a piece to the puzzle of chronic illness and need to be tackled in the proper order.

If you’d like help managing retroviral activity, Lyme disease, and chronic illness, my At-Home Program could give you the guidance you need to restore your vibrant health and energy.

Plus, you’ll get a free antiviral bioactive carbon supplement when you sign up.

Additionally, the doctors in my 1:1 Coaching Program can provide you
with the personal touch and customized protocols that lead you to your health and wellness goals.

11 Odumade, Oludare A et al. “Progress and Problems in Understanding and Managing Primary Epstein-Barr Virus Infections.” Clinical microbiology reviews, vol. 24, no.1, Jan 2011. Web

12 “Viruses That Can Lead to Cancer.” cancer.org, American Cancer Society, n.d. Web

13 Flavin, Dana. “Reversing Splenomegalies in Epstein-Barr Virus-Infected Children: Mechanisms of Toxicity in Viral Diseases.” semanticscholar.org, Allen Institute for Artificial Intelligence, 2006. Web

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Parkinson’s A Second Look

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The Ruggiero-Klinghardt (RK) Protocol for the Diagnosis and Treatment of Chronic Conditions with Particular Focus on Lyme Disease

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Clinical Procedures

The Ruggiero-Klinghardt (RK) Protocol for the Diagnosis and Treatment of Chronic Conditions with Particular Focus on Lyme Disease

Dietrich Klinghardt and Marco Ruggiero

Sophia Health Institute and Klinghardt Academy, Woodinville, WA., USA

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Article history

Received: 20-02-2017 Revised: 28-02-2017 Accepted: 08-03-2017

Corresponding Author:
Marco Ruggiero
Sophia Health Institute and Klinghardt Academy, Woodinville, WA., USA
Email: marco.drruggiero@gmail.com

Abstract: Here we describe the Ruggiero-Klinghardt (RK) Protocol that is based on integration of Autonomic Response Testing (ART) with diagnostic ultrasonography and on application of therapeutic ultrasounds; the latter are used as a provocation tool and as an instrument to optimize drug uptake and utilization in specific areas of the body. This protocol consists of a precise sequence of diagnostic and therapeutic procedures with the ultimate goal of improving sensitivity and specificity of diagnosis at the same time evaluating and optimizing efficacy of treatments in chronic conditions including, but not limited to, persistent Lyme disease. The RK Protocol represents a paradigm shift in diagnostics and therapeutics: Thus, compartmentalized microbes, transformed cells, toxins and metabolites could be detected using a safe and non-invasive method. In addition, the RK Protocol allows optimization of efficacy of drugs and other therapeutic interventions. Although the RK Protocol was initially developed for persistent Lyme disease, it shows significant potential in conditions ranging from cancer to neurodegenerative diseases and autism. In oncology, the RK Protocol may serve to facilitate early diagnosis and to increase sensitivity of cancer cells to the killing effects of a variety of remedies ranging from conventional radio- and chemotherapy to more recent forms of immunotherapy. Thus, the 1st goal of the RK Protocol is diagnostic: That is, to make pathogens, toxins, transformed cells and cells infected by viruses that are inaccessible to conventional diagnostic and therapeutic tools, “visible” to the therapist who can detect them with laboratory methods and deal with them with appropriate interventions; and also to make them “visible” to the immune system that can fight them in a physiological manner. The 2nd goal is to optimize drug uptake and utilization in the organs and tissues studied and targeted with these procedures.

Keywords: Lyme, Ultrasound, Autonomic Response Testing, Immune System, Imaging, Brain

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Introduction

The infectious disease known as Lyme borreliosis, or Lyme disease, is the most common infection due to tick bites and sometimes also to other stinging insects in the Northern Hemisphere. Although estimates vary and it is likely that the number of cases worldwide is much higher, there is general consensus that the disease affects hundreds of thousands of individuals a year in North America, Europe and northern Asia with incidence of the infection on the rise (Shapiro, 2014;

Gingrich et al., 2001). The designation “Lyme disease” derives from the small New England villages of Lyme and of Old Lyme where the arthritic manifestations of the disease were first described in 1975 as “a rather random clustering of several cases of juvenile chronic arthritis” (Burmester, 1993).

The etiologic infectious agent, Borrelia burgdorferiwas identified by Wilhelm “Willy” Burgdorfer it in 1981 and, since the original observation limited to arthritis, the clinical spectrum of the infection now includes a variety of clinical conditions such as erythema migrans,

American Journal of Immunology

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© 2017 Dietrich Klinghardt and Marco Ruggiero. This open access article is distributed under a Creative Commons Attribution (CC-BY) 3.0 license.

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acrodermatitis chronica atrophicans, lymphadenosis benigna cutis, arthritis, myocarditis, neuroborreliosis (meningo-encephalitis, meningo-radiculitis, meningitis), myositis and various ocular and skin disorders (Burgdorfer, 1991; Chomel, 2015). Interestingly, as knowledge of the disease deepened, the increase in the number of clinical manifestations of the infection was paralleled by the increase in the number of spirochete bacteria belonging to the genus Borrelia associated with the disease; Borrelia burgdorferi sensu stricto is found in the Americas whereas Borrelia afzelii and Borrelia garinii, in addition to Borrelia burgdorferi, are observed in Europe and Asia (Chomel, 2015). In a sort of an exponential increase in complexity, it was later discovered that the ticks responsible for transmitting theBorreliae that are Ixodes scapularis and Ixodes pacificus, also have the potential to transmit an increasingly expanding list of other pathogenic microbes that include bacteria, viruses and parasites such asAnaplasma phagocytophilum, Babesia microti, deer tick (Powassan) virus, Borrelia miyamotoi and the Ehrlichia muris-like organism (Caulfield and Pritt, 2015). Obviously, the presence of coinfections renders the treatment and the diagnosis rather difficult and contributes to patient morbidity and mortality as well as to the appearances of the so called “post-treatment Lyme disease” also known as “chronic Lyme” or, as we prefer to denominate it in this study, “persistent Lyme disease”. Thus, persistence of Borrelia burgordferi in tissues after efficient antibiotic treatment has been demonstrated in a variety of experimental models that include primates, but there is currently no affordable, non-invasive, method to detect specific persistent microbes (or their metabolites) in vital organs such as the brain, spinal chord or heart (Straubinger et al., 1997; 2000; Hodzic et al., 2008; Yrjänäinen et al., 2010; Embers et al., 2012).

Persistent Lyme is a diagnosis that is given to patients with prolonged, unexplained symptoms, that may be severe, occurring after efficient antibiotic treatment. Such symptoms are most often non-specific and include neurological symptoms such as chronic fatigue, difficulty to sleep, inability to focus, irritability, depression, headache and dizziness; gastrointestinal symptoms such as abdominal pain, nausea and diarrhea; symptoms suggestive for local or systemic inflammation such as pharyngodynia, enlarged lymphnodes, muscle rigidity, myalgia and arthralgia (Feder et al., 2007). The non-specificity of these symptoms and the absence of reliable laboratory tests has led to questioning the very existence of a persistent Lyme disease since it is claimed that there is no strict evidence that the chronic post- treatment symptoms are attributable to ongoing infection with Borrelia burgdorferi or with any other identified organism (Feder et al., 2007; Halperin, 2015). However, despite the controversies surrounding the existence, the

definition or the nature of persistent Lyme, it is indisputable that this condition leads to significant sequelae that dramatically decrease the quality of life of affected patients. Thus, a recent study from the John Hopkins School of Medicine describes the physical and social limitations associated with the condition that lead to fundamental changes in the way of living. In this study, the Authors evidence the disease-specific factors that contribute to symptom and illness invisibility and the pervasive medical uncertainty regarding persistent Lyme that promotes an increased sense of personal responsibility for care. The Authors conclude that “similar to other contested or medically unexplained syndromes, our findings suggest that the social sequelae of post-treatment Lyme disease/chronic Lyme can be equally protracted as the physical effects of this illness” (Rebman et al., 2015).

The main reason for the contested status of persistent Lyme as a fully recognized disease mainly resides in the objective difficulty to assess the persistence of Borrelia, or other pathogens responsible for coinfections, after efficient antibiotic treatment. However, persistence ofBorrelia burgordferi in tissues after antibiotic treatment has been demonstrated in a variety of experimental models, which include primates, that were infected withBorrelia burgdorferi and then received aggressive and successful antibiotic treatment for 4-6 months (Straubinger et al., 1997; 2000; Hodzic et al., 2008; Yrjänäinen et al., 2010; Embers et al., 2012).

The latter study demonstrates that the pathogen is able to resist antibiotic treatment in primates, even though the Borrelia in itself is not known to possess resistance mechanisms and it is susceptible to common antibiotics such as doxycycline and ceftriaxone in vitro. Therefore, mechanisms other than classic antibiotic resistance have been hypothesized to explain the persistence of the bacteria and these include the formation of drug-tolerant persister cells (Sharma et al., 2015), cell wall deficient forms and biofilm residing forms, as well as the generation of cystic forms of Borrelia with low metabolic activity that enables the spirochete to survive in a hostile environment until conditions are favorable to proliferate again (Murgia and Cinco, 2004).

The controversies and the difficulties associated with the diagnosis and treatment of persistent Lyme could be resolved by the existence of reliable and reproducible tests that were able to ascribe the symptoms to chronic infections rather than to a wide number of other conditions that may present themselves with similar or identical symptoms (Eshoo et al., 2013; Curcio et al., 2016).

At Sophia Health Institute, we use a manual biofeedback technique, denominated Autonomic Response Testing (ART) that has the goal of assessing

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the presence and/or the persistence of spirochete and other infectious agents that may be associated with persistent Lyme disease. ART represents an evolution and an expansion of the technique that was originally proposed by Omura (1981) and subsequently validated in a number of studies including two randomized-order blinded studies registered as a clinical trial (Jacobs et al., 1984; Jensen et al., 2016). In a paper published in 2016, ART developed at the Sophia Health Institute was utilized by independent researchers in the diagnosis and treatment of painful scars. In this study, the Authors state that “In our experience, ART produces useful and consistent information most of the time” (Chung and LaRiccia, 2016). The validity of ART was further confirmed in another independent investigation focused on breast cancer (Chung and LaRiccia, 2017).

In addition to ART, at Sophia Health Institute, we investigate the presence of pathogens in urine samples of patients using Polymerase Chain Reaction (PCR) in order to identify genes specific for microbes known to be associated with persistent Lyme. However, we noticed quite often that, while ART suggested the presence of pathogens, such a presence was not confirmed by the PCR-based DNA test performed on urine samples. Other researchers have noticed the low sensitivity of the urine DNA test when it is performed without the provocation method that will be described in the following sections of this study. However, serendipitous observation at Sophia Health Institute suggested that the results of the urine DNA test matched more closely the results of ART when urine samples were collected after the patient had received a deep tissue massage with focus on the symptomatic body regions; massage that had been performed to relieve tension or for other reasons. Based on these observations and in search for a reliable method to improve the sensitivity of the urine DNA test, we decided to apply pulsed therapeutic ultrasounds focused on critical areas to mimic or improve the effects of deep tissue massage. This approach is based on the widely recognized feature of ultrasounds to transmit waves of compression and relaxation in biological tissues with resulting changes in organ, cellular and molecular structures that can be exploited in the context of therapy (Leinenga et al., 2016). Thus, we have recently demonstrated that non-thermal ultrasounds induce quasi-instantaneous changes in human neurons and murine microglial cells in vitro with results that are consistent with the observed effects of ultrasounds on mental states (Cosentino et al., 2015; Bocchi et al., 2015; Hameroff et al., 2013).

Here we describe an original protocol denominated Ruggiero-Klinghardt (RK) Protocol that is based on ART and on a precise sequence of diagnostic and therapeutic ultrasounds for the accurate and reliable

diagnosis and treatment of persistent Lyme disease and other chronic conditions.

Materials and Methods

Ultrasound Systems

For diagnostic ultrasonography, we used a portable MicroMaxx ultrasound system manufactured by Sonosite, Bothell WA, USA, with color-doppler application and with a linear (L38e) and a convex (C60e) transducer. This system is approved for many applications including cephalic (brain) imaging and has the same features of the system that one of us (M.R.) had previously used to characterize the lesions in the brains of autistic children (Bradstreet et al., 2014); in this latter study, the safety of the procedure is thoroughly described. In order to avoid any bias, the images were not exported and pictures of the screen of the ultrasound system were taken with the camera of a common smartphone. This procedure was chosen in order to avoid the possibility of editing the images, that are presented here exactly as they were produced; this procedure, however, inevitably leads to poor quality images since these were taken at the bedside without any consideration for professional photo-shooting. An example can be observed in Fig. 4, where portions of the screen of the ultrasound system can be observed. The pictures of the images were then copied in a Power Point file in order to add captions, arrows or circles that are useful to show the anatomical structures that were studied and their alterations. The application of therapeutic ultrasounds, that is an essential part of the RK Protocol, was performed using an Intelect TranSport Ultrasound Therapy device (Chattanooga Medical Supplies Inc., Chattanooga TN, USA). This system enables pulsed and continuous operation (10, 20, 50 and 100%) on 1 and 3.3 MHz frequencies using a 5 cm2 sound head applicator.

Urine DNA Test

Urine samples were collected before and after application of therapeutic ultrasounds at Sophia Health Institute according to the protocol summarized in Table 1 and following the recommendations of the company performing the DNA test. The test was performed by DNA Connexions, Colorado Springs CO, USA. The Lyme panel tests for 4 different genes that are found inBorrelia burgdorferi and 8 common Lyme disease co- infectors including Babesia microti, Babesia divergens,Babesia duncani, Bartonella bacilliformis, Bartonella henselae, Bartonella quintanta, Borrelia miyamotoi,Borrelia recurrentis, Ehrlichia chaffensis andAnaplasma phagocytophilum. According to the company and consistent with common knowledge, a positive PCR- based Lyme test indicates the presence of DNA fromBorrelia burgdorferi and/or other co-infectors.

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Table 1. Step 1. Step 2. Step 3. Step 4.

Step 5.

The Ruggiero-Klinghardt (RK) Protocol steps of diagnostic and therapeutic procedures
First comprehensive Autonomic Response Testing (ART).
Total-body diagnostic ultrasonography that has the role of further refining the diagnostic hypotheses put forward by ART. Application of therapeutic ultrasounds with particular focus on the areas identified as “abnormal” with the previous steps. Second ART performed after application of therapeutic ultrasounds: Comparison of the results with those obtained with the first ART of Step 1.
Collection of midstream urine in a sterile container that is then shipped to the laboratory. Alternatively, the presence of microbes, toxins, circulating cancer cells or other pathogenic noxae is investigated in other biological matrixes such as stools, blood or serum or breath that are appropriately collected.
Pharmacological treatment with remedies specific for the identified pathogens.
Daily application of therapeutic ultrasounds targeted to the areas identified with the previous steps.

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Step 6.
Step 7.
Follow-up at 3-4 month intervals to evaluate the effectiveness of treatment and to assess the treatment end-point.

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A negative result, however, does not prove that a patient is not infected with a tick borne infection, rather it indicates the absence of detectable DNA pertaining to microbes associated with Lyme and/or other tick borne co-infections in that particular urine sample. Stage of infection, timing of courses of antibiotics and persistence of latent microbes in reservoirs or sanctuaries, are only some of the factors that may affect the detectability of the DNA of spirochetes in urine samples.

Autonomic Response Testing (ART)

ART, invented and developed by one of us (D.K.), represents an evolution and an expansion of the test originally described by Omura (1981).

At variance with the test proposed by Omura, ART takes into account the entirety of the autonomic response and not only the strength or the resistance of muscles. This is particularly important in the study of parasympathetic activity and in the evaluation of the balance between sympathetic and parasympathetic activities. A core principle of the test relies on the “Resonance Phenomenon Between Identical Substances”. Thus, a culture of a particular pathogen is used to non-invasively detect the presence of this very pathogen in a particular body region or organ. A description of ART can be found in a recently published peer-reviewed study (Chung and LaRiccia, 2016). In the present study, as per the RK Protocol, ART was performed two times, i.e., before and after application of therapeutic ultrasounds as summarized in Table 1; the results of the two tests were then compared and recorded. Since interpretation of ART can provide information on the potential responsiveness of the patient to different therapeutic approaches and since the involvement of the immune system in persistent Lyme is widely acknowledged, we used an emulsion of chondroitin sulfate, vitamin D3 and oleic acid endowed with immune modulating properties as positive control (dr. reinwald healthcare, Schwarzenbruck, Germany). The characteristics of this emulsion and its potential use in integrative immunotherapy have been recently described (Schwalb et al., 2016).

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Results

Description of the RK Protocol Step 1

After having collected a careful anamnesis and critically reviewed clinical records, laboratory exams and radiological images, the patient is invited to empty her/his bladder before performing the first step of the RK Protocol that consists in ART which is performed by the therapist with the help of an assistant; the sequence of steps of the RK Protocol is summarized in Table 1. ART provides the initial information that is useful to restrict the spectrum of diagnostic hypotheses and to identify the organs or the areas of the body that need further investigation.

Step 2

Then, as the second step of the protocol, a diagnostic total body ultrasonography is performed with particular focus on those areas that have tested positive with ART.

The following examples elucidate the synergy between ART and ultrasonography in diagnostics. Figure 1 shows the ultrasonographic appearance of the submandibular salivary gland of a subject who had tested positive with ART in that area. The inhomogeneous appearance of the gland with irregular hypoechoic areas is indicative of a diffuse inflammatory process whose origin (viral or autoimmune) requires further investigation. Figure 2, shows an enlarged and possibly inflamed, deep cervical node in a subject with symptoms of neuroborreliosis who had tested positive with ART. The blood vessels in the hilum of the node are clearly visible at the echo-color-doppler and their appearance is consistent with a condition of hyper-afflux. It is worth noticing that inflammation of the deep cervical nodes may be associated with impaired lymphatic drainage from the brain lymphatic system (also known as “glymphatic system”) with consequent stagnation of lymph in the brain and accumulation of metabolites and neurotoxins in addition to potential disruption of

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the brain microbiota. These events may be associated with, if not responsible for, some of the symptoms of neuroborreliosis, autism and other neurological diseases (Bradstreet et al., 2014; Ruggiero, 2016). Figure 3, shows the appearance of the vagus nerve in a subject who had tested positive with ART for parasympathetic imbalance. In this transversal projection, the vagus nerve appears as a small triangular structure located posteriorly inside the carotid sheath between the common carotid artery and the internal jugular vein; it shows an internal honeycomb structure. In this subject, the epineurium appears as a thickened hyperechoic ring surrounding the nerve. Figure 4, shows the appearance of the thyroid in longitudinal projection in a subject who had tested positive with ART for parathyroid involvement. The arrow indicates a roundish area that protrudes from the posterior margin of the thyroid and could be interpreted as an enlarged parathyroid gland. Please notice that the solid arrow was inserted during the ultrasound examination whereas the dotted circle was inserted during the preparation of the figure, for the sake of clarity, using a Power Point program.

Step 3

After having performed the diagnostic total body ultrasonography, the third step of the RK Protocol is then performed; therapeutic ultrasounds are directed toward those organs or areas of the body where clinical suspicion of inflammation, proliferative, degenerative or infectious diseases is present. The rationale for this procedure is to exploit the mechanical effects of ultrasounds in human tissues with the goal of mobilizing pathogens, toxins, transformed cells, cells infected by viruses, or cells of

the immune system carrying pathogens, toxicants or antigens of transformed cells, so that they may be identified by the second ART and by specific tests such as the urine DNA test. In other words, the goal of application of therapeutic ultrasounds is to force the exit from reservoirs or sanctuaries where pathogens or transformed cells are invisible to the immune system and to the therapist performing ART or using laboratory methods and are protected from therapeutic intervention (Cory et al., 2013). Choice of the pulsed sequence and frequency depends on the location of the organ to be treated.

For example, when treating the spleen, a major organ of the immune system, we choose a pulsed sequence indicated as 50% and a frequency of 1 MHz. First the spleen is identified by diagnostic ultrasonography (Fig. 5) and then it is treated for 3 min, slowly moving the sound head applicator so to send the ultrasound waves to most areas of the organ. The effectiveness of application of therapeutic ultrasounds is then assessed by studying the blood flow in the spleen after the treatment, using for this purpose the diagnostic echo-color-doppler technique. As shown in Fig. 6, immediately after application of therapeutic ultrasounds, a significant increase in blood flow can be observed at the diagnostic ultrasonography. We have previously demonstrated that such an increase corresponds to the activation of cells of the immune system inside the spleen, with particular reference to macrophages (Ruggiero et al., 2014). Thus, the mechanical waves of compression and relaxation of therapeutic ultrasounds “squeeze” the organ, presumably at the level of the microscopic anatomy of spleen as well as at the level of cellular and molecular structures such as the proteins of the cytoskeleton (Hameroff et al., 2013).

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Fig. 1. Ultrasonographic appearance of the submandibular salivary gland118

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Fig. 2. Ultrasonographic appearance of a deep cervical node

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Fig. 3. Ultrasonographic appearance of the vagus nerve

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Fig. 4. Ultrasonographic appearance of the thyroid119

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Fig. 5. Ultrasonographic appearance of the spleen before therapeutic ultrasound treatment

Fig. 6. Blood flow in the lower pole of the spleen before and after application of therapeutic ultrasounds

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We hypothesize that mobilization of pathogens or toxins from sanctuaries or reservoirs is followed by their elimination through the urine and this phenomenon should optimize the sensitivity of the urine DNA test. In addition, we have observed that the sensitivity and the specificity of ART significantly increase after application of therapeutic ultrasounds, thus improving the intra- and inter-operator reproducibility of the technique. Furthermore, as we have observed using the immune modulating emulsion mentioned in the Materials and Methods, application of therapeutic ultrasounds to a specific organ significantly improves the sensitivity of the organ to the remedy. Such an effect did not come as a surprise since it is known that ultrasounds have the potential to increase the effectiveness of a number of drugs and remedies by favoring their cellular uptake and overcoming the barriers that prevent delivery of drugs to specific lesions (Bui et al., 2017).

We observed that the biological effects of application of therapeutic ultrasounds were particularly remarkable when the technique was applied to the brain, with particular reference to the temporal lobes. We and others

have previously demonstrated that ultrasounds could be directed toward specific areas of the brain using carefully selected acoustic windows such as the temporal squama (Ruggiero et al., 2013; Hameroff et al., 2013; Bradstreet et al., 2014).

More recently, we have demonstrated that ultrasounds elicit cellular and molecular responses in neurons and glial cells that are consistent with their effects on mental states and can be exploited in the context of therapy (Cosentino et al., 2015; Bocchi et al., 2015; Hameroff et al., 2013; Leinenga et al., 2016). Based on these evidence, we incorporated transcranial ultrasonography in the RK Protocol. Thus, after having performed the first ART of step 1, a diagnostic ultrasound scan of the brain is performed as shown in Fig. 7. This figure shows the squama of the temporalis bone as a homogeneous hyperechoic structure; the meninges are visualized as a series of alternating hyper- and hypoechoic layers with a regular structure; the subarachnoid space appears as an anechoic line due to the presence of extra-axial fluid; and the gray matter of the temporal lobe (in this case, the right temporal lobe)

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appears hypoechoic in comparison with the meninges showing a layered structure that corresponds to the cellular architecture of the cerebral cortex described by von Economo and Koskinas (1925). Study of the subarachnoid space may be used to evaluate the accumulation of extra-axial fluid according to the technique described in Bradstreet et al. (2014). However, at variance with our previous studies, at Sophia Health Institute, we do not limit our intervention to the evaluation of extra-axial fluid accumulation and how it relates to neurological symptoms, but we use that information to proceed with application of therapeutic ultrasounds to the brain and the deep cervical nodes (Fig. 2) with the intent of restoring the flow of cerebral lymph and possibly ameliorating neurologic symptoms when present (Bradstreet et al., 2015; Tarasoff-Conway et al., 2015).

In order to reach this goal, the deep cervical nodes that have been visualized by diagnostic ultrasonography are treated with therapeutic ultrasounds using a pulsed sequence indicated as 20% and a frequency of 3.3 MHz for 90 sec on each side of the neck. Only after having treated the nodes, the brain is treated with therapeutic ultrasounds that are administered through the temporal acoustic window using a pulsed sequence indicated as 10% and a frequency of 3.3 MHz for 90 sec on each side of the head. The rationale for this sequence of therapeutic procedures lays in the consideration that inflamed deep cervical nodes may pose an obstacle to the efflux of the lymph from the brain and, therefore, it is indicated to treat the deep cervical nodes first in order to reduce inflammation by exploiting the anti-inflammatory effects of pulsed ultrasounds (Jia et al., 2016). Subsequent treatment of the brain with therapeutic ultrasounds thus sends mechanical pressure waves consisting of alternate compression and relaxation that may favor the circulation of lymph in the brain

lymphatic system and the removal of catabolites and toxins that may have become stagnant in the presence of an obstacle to the circulation of lymph (Raper et al., 2016). It is worth noticing that, given the anatomical proximity of the two structures (Fig. 2 and 3) application of therapeutic ultrasounds to the deep cervical nodes may correspond to treatment of the vagus nerve as well. Thus, it is well assessed that vagus nerve stimulation provides a number of benefits ranging from treatment of affective disorders in psychiatry (Cimpianu et al., 2017) to improvement in recovery from traumatic brain injury (Neren et al., 2016). Not surprisingly, the second ART, performed after each therapeutic ultrasound treatment, showed changes consistent with the effects described above.

Step 4

The fourth step of the RK Protocol consists in the second ART that is performed after application of therapeutic ultrasounds and in the comparison of the results with those obtained with the first ART of Step 1.

Step 5

The fifth step of the RK Protocol consists in the collection of the urine samples that has to be performed after the application of the therapeutic ultrasounds; the patient collects the naturally occurring next midstream urine in the sterile container provided by the laboratory performing the urine DNA test and the sample is shipped overnight to the laboratory. Although in this study we describe the results obtained with the urine DNA test, the presence of microbes, toxins, circulating cancer cells or other pathogenic noxae can be investigated in other biological matrixes such as stools, blood or serum or breath that are appropriately collected and analyzed by specialized laboratories.

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Fig. 7. Ultrasonographic appearance of the brain at the level of the right temporal lobe121

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Step 6

Successively, as the sixth step of the RK Protocol, the patient is treated with specific remedies that are targeted, for example, toward the pathogens identified with the previous steps. When antimicrobials are used, their choice is determined by applying common current understanding and fine tuning the selection with ART.

Step 7

The seventh step of the RK Protocol is performed in conjunction with the previous one and consists in the targeted application of therapeutic ultrasounds; such a procedure is performed once daily in the same manner described above in order to exploit the known therapeutic effects of pulsed ultrasounds that comprise anti-inflammatory effects, enhanced lymphatic drainage and optimization of drug uptake and utilization.

The RK Protocol is then repeated after three to four months in order to evaluate the effectiveness of treatment and to assess the treatment end-point.

Discussion

In this study we describe for the very first time a protocol, the RK Protocol, that is based on the integration of ART, as a screening tool and ultrasounds; the latter are utilized as a diagnostic tool, as a provocation tool and as a means to optimize drug uptake and utilization in specific areas of the body. This protocol consists of a precise sequence of diagnostic and therapeutic procedures with the ultimate goal of improving sensitivity and specificity of diagnosis and evaluating and optimizing efficacy of treatments in chronic conditions including, but not limited to, persistent Lyme. The sequence that we have developed is summarized in Table 1 and comprises, as a first step, a comprehensive ART screening, followed, as a second step, by total-body diagnostic ultrasonography that has the role of further refining the diagnostic hypotheses put forward by ART. Diagnostic ultrasonography also has the role to precisely identify the anatomic localization of the organs or the structures toward which the pressure waves of the therapeutic ultrasounds will then be directed in the third step of the protocol.

Application of therapeutic ultrasounds, the third step, plays three roles in the RK Protocol:

  • It is used to exploit the well-known therapeutic effects of ultrasounds that have been observed in a variety of conditions associated with chronic inflammation or neurodegeneration (Jia et al., 2016; Leinenga et al., 2016)
  • It is used to increase the potential effectiveness of remedies, an effect that can be further verified by the second, post-ultrasound ART (step 4). This

phenomenon is due to the known efficacy of therapeutic ultrasounds to increase the uptake and utilization of remedies in cells and tissues (Bui et al., 2017)

• More specific to the protocol, it is used to force the exit of pathogens, toxins, transformed cells, cells infected by viruses and cells of the immune system that have interacted with all of the above, from tissue reservoirs or sanctuaries so to render them “visible” to the immune system and to the therapist who will then perform the fourth and fifth steps of this protocol

These are, respectively, the second ART screening performed after application of therapeutic ultrasounds (fourth step); and collection of samples for the molecular detection of microbes, toxins, circulating cancer cells etc. that can be detected in urine, stools, blood or serum, breath or other biological matrixes (fifth step).

We have observed that the results of the second ART, performed after application of therapeutic ultrasounds, are significantly different from those of the initial first ART. For example, signs that could be interpreted as the presence of pathogens associated with coinfections of persistent Lyme that had not been detected by the initial first ART exam, became apparent when the second ART was performed after application of therapeutic ultrasounds, thus improving the diagnostic sensitivity of the procedure. We noticed that also the inter- and intra- variability of ART results were significantly decreased when the test was performed after application of therapeutic ultrasounds.

A major advantage of the RK Protocol consists in the possibility of validating the results obtained through the first steps using specific diagnostic methods that are based on molecular biology. In the case of persistent Lyme disease, for example, we used the urine DNA test that identifies genes pertaining to agents known to infect patients with persistent Lyme. Figure 8, shows a significant example of the results of the urine DNA test performed before and after application of therapeutic ultrasounds. The case of this patient with a history of angina that could not be causally diagnosed with the classic cardiology tests (such as stress echo etc.) is shown as a paradigmatic example representing a large majority of patients whom we have observed during the implementation of the RK Protocol at Sophia Health Institute. In this patient, urine samples were collected before and after application of therapeutic ultrasounds following the RK Protocol. In the urine sample collected before application of therapeutic ultrasounds (Fig. 8, left panel) the PCR-based DNA analysis did not reveal the presence of any pathogen among those detected by the Lyme panel even though the patient had tested positive for the presence of Bartonella species with ART. In

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addition, this patient had tested negative for the presence of antibodies against Bartonella henselae (IgG/IgM) and negative also in the Western Blot test (CDC criteria) from the IgeneX lab in the blood draw obtained the same day before the application of the RK Protocol. In other words, before the application of therapeutic ultrasounds, this patient was one of the many cases where ART provided positive results that could not be confirmed by the urine DNA test or by antibody-based blood tests. However, the DNA test performed on the urine sample collected one hour after application of therapeutic ultrasounds, clearly shows the presence of one well- identified pathogen, Bartonella bacilliformis, a well- known cause of endocarditis, commonly diagnosed only post-mortem, whose presence, albeit suggested by ART, was not evident before the application of the ultrasounds (Fig. 8, right panel).

In proceeding with the implementation of the RK Protocol, the patient responded rapidly and favorably to targeted biological treatment involving daily therapeutic ultrasound application and several anti-microbial agents.

In our opinion, these results represent a paradigm change in diagnostics and therapy. Thus, the RK Protocol, enables to reach a clear diagnosis in cases that otherwise would have been labeled as “uncertain” or “undetermined” because of the discrepancy between ART and laboratory results. Quite obviously, a diagnosis based on the concordance of ART and laboratory results

enables the therapist to implement specific therapeutic approaches that would have not been possible without the RK Protocol.

Although the RK Protocol was initially developed for persistent Lyme, its potential in other conditions ranging from cancer to neurodegenerative disorders did not escape our attention. For example, in the field of oncology the RK Protocol may serve as a tool for early diagnosis and as an instrument to increase the sensitivity of cancer cells to the therapeutic effects of a variety of approaches ranging from conventional radio- and chemotherapy to more recent forms of immunotherapy (Schwalb et al., 2016). In some aspects, the RK Protocol reminds the “shock and kill” strategy that is being pursued to eliminate HIV reservoirs that are responsible for the latency and persistence of the virus. This strategy aims at inducing HIV replication in latent viral reservoir; although this concept may appear counterintuitive since the goal of antiretroviral therapies should be to inhibit, not to stimulate, viral replication, the rationale is to make the virus “visible” to the immune system and to the antiretroviral drugs (Melkova et al., 2017). Analogously, the goal of the RK Protocol is to make pathogens, toxins, transformed cells and cells infected by viruses that are inaccessible to diagnostic and therapeutic tools, “visible” so that they can be recognized and dealt with, both by the therapist and the immune system.

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Fig. 8. Example of urine DNA test for the detection of Lyme infection and co-infections123

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Thus, the RK protocol may find notable applications in the diagnosis of localized infections such as Lyme carditis (Robinson et al., 2015) or infections of the brain by Babesia (Aikawa et al., 1992). In these cases, that are notoriously difficult to diagnose, the RK Protocol has the additional benefit of allowing the localization of the infectious agents without resorting to invasive procedures. To this end, the therapeutic ultrasound treatment is applied to the areas of the body where the presence of the infectious agent is suspected and, if the diagnostic hypothesis is correct, the urine DNA test would show the presence of the pathogen only after application of therapeutic ultrasounds to specific areas. For example, if the presence of Babesia became evident only after application of therapeutic ultrasounds to the brain and not before, it could be concluded that the pathogen resided in the brain and was mobilized by the pressure waves generated by the therapeutic ultrasounds.

Therefore, the RK Protocol leads to profound new possibilities: Compartmentalized microbes, hidden transformed cells, toxins and metabolites could be detected using a safe and non-invasive method. In addition to these exciting possibilities in the field of diagnostics, the RK Protocol allows optimization of the therapeutic efficacy of drugs administered parenterally; thus, application of therapeutic ultrasounds before administration of drugs specifically targeted to microbes or toxins detected by the diagnostic steps of the protocol, would significantly increase uptake and utilization of the drugs themselves with a significant improvement of their therapeutic efficacy. ART would thus have the role of narrowing down the number of diagnostic hypotheses and therapeutic options with a resulting decrease of unnecessary, expensive and time-consuming tests.

Conclusion

We have developed a novel protocol for the non- invasive diagnosis and treatment of persistent Lyme and other chronic conditions due to persistent infections, toxicities, neoplastic transformation or neurodegeneration. The RK Protocol offers the advantage of being safe, rapid and relatively inexpensive and it can be easily implemented in any health institution whether in the developed world or elsewhere, without the need for sophisticated and expensive instruments. This protocol aims at achieving accurate and early diagnosis, at indicating the most appropriate therapeutic intervention and at maximizing the efficacy of specific therapeutic interventions.

Acknowledgement

The Authors wish to thank Ms. Daniela Deiosso for inspiring discussion and relentless support and the therapists at the Sophia Health Institute for their precious collaboration.

Author’s Contributions

Dietrich Klinghardt: The inventor and developer of ART and performed all the diagnostic and therapeutic procedures described in this study.

Marco Ruggiero: Developed the ultrasound-based techniques described in this study, wrote the first draft of this paper, provided critical input and assisted in revising and improving the paper. He had no type of involvement in the treatment of patients.

Conflict of Interest

Dietrich Klinghardt is the inventor of ART and the founder of the Klinghardt Institute, the Klinghardt Academy, the Institut fuer Neurobiologie and the Sophia Health Institute, a private clinic. Dr. Klinghardt consults for several companies producing supplements and other remedies that, however, are not mentioned in this study. Marco Ruggiero is consultant for the company “dr. reinwald healthcare”, that provided the emulsion of chondroitin sulfate, vitamin D3 and oleic acid mentioned in this study and he is the founder and CEO of the Swiss company Silver Spring Sagl, a company that produces supplements and probiotics. None of the products of Silver Spring Sagl is mentioned in this study. Marco Ruggiero is member of the Editorial Board of The American Journal of Immunology and is waived from the Article Processing fee for this contribution; he receives no remuneration for his editorial work.

Ethics

This article is original and contains unpublished material. The corresponding author confirms that the other author has read and approved the manuscript.

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This is the first published clinical case of cancer: lifted with imuno and Bravo in 3 months.

imuno-and-Bravo-Myeloma-Cured-18Jun19

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Original Research Paper

Use of an Extremely Biodiverse Probiotic and a Supplement Based on Microbial Chondroitin Sulfate is Associated with a Significant Decrease of Serum Free Kappa Light Chains as well as a Trend Toward Normalization of Kappa/Lambda Ratio and of Plasma Cell Bone Marrow Infiltration in a Case of Multiple Myeloma

1Nicola Antonucci, 2Stefania Pacini and 2Marco Ruggiero1Biomedical Centre for Autism Research and Treatment, Bari, Italy

2Silver Spring Sagl, Arzo-Mendrisio, Switzerland

American Journal of Immunology

Article history

Received: 27-05-2019 Revised: 13-06-2019 Accepted: 18-06-2019

Corresponding Author: Marco Ruggiero
Silver Spring Sagl, Via Raimondo Rossi 24, Arzo- Mendrisio 6864, Switzerland info@bravo-europe.com

Introduction

Multiple myeloma is a neoplasia of plasma cells present in roughly 3-4% of the population over the age of 50 years and characterized by a cytogenetically heterogeneous plasma cell population. Multiple myeloma is considered an incurable hematologic neoplasia often requiring multiple sequential therapeutic regimens that have to be constantly tailored to the changing patient’s conditions (Rajkumar et al., 2016). The standard of care for patients over 65 years and/or patients with serious concurrent chronic diseases is represented by chemotherapy with prednisone and melphalan or, more recently, with proteasome inhibitors (Hungria et al., 2019). An obstacle to chemotherapy of multiple myeloma is represented by renal insufficiency, a condition that is common in these patients and can often limit the choice of therapy (Mikhael et al., 2018).

Here we describe the case of a 71-year-old woman with multiple myeloma associated with severe chronic renal insufficiency who, since October 2018, assumed a novel, extremely biodiverse, probiotic and a

Abstract: We describe the case of a 71-year-old woman with multiple myeloma associated with severe chronic renal insufficiency who, since October 2018, assumed a novel, extremely biodiverse, probiotic and a supplement based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine; such a nutritional regimen was associated with a dramatic decrease of serum free Kappa light chains (from 2,190 to a minimum of 119.40 mg/L) as well as a trend toward normalization of Kappa/Lambda ratio (from 187 to a minimum of 3.16) and of plasma cell bone marrow infiltration (from 20 to 5%). supplement based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine; such a nutritional regimen was associated with a dramatic decrease of serum free Kappa light chains as well as a trend toward normalization of Kappa/Lambda ratio and of plasma cell bone marrow infiltration.

Case Presentation

The patient, a woman born in 1948, had been diagnosed with monoclonal gammopathy in 2010; in 2012 she developed acute renal insufficiency treated with dialysis and plasmapheresis. This event was followed by deep venous thrombosis with occlusion of the iliac- femoralis and femoralis-popliteal axes and systemic hypertension. Chronic renal insufficiency with frequent episodes of acute relapses resulted in extensive glomerular-tubular damage as evidenced by urinary protein electrophoresis; a renal biopsy documented advanced glomerulosclerosis with severe interstitial damage. In October 2018, the patient was admitted at the Oncohematological Department of the San Matteo hospital of Pavia, Italy; the document released by the hospital on October 29, 2018, reports that bone marrow biopsy of the posterior iliac crest confirmed a diagnosis of multiple myeloma with monotypical plasma cell bone marrow infiltration accounting for 20% of total cells. Study of serum free light chains showed significant alteration of the Kappa/Lambda ratio (free Kappa: 2,190 mg/L. Free Lambda: 11.7 mg/L. Ratio Kappa/Lambda: 187) that was consistent with the severity of the oncohematological disease according to the updated criteria for the diagnosis of multiple myeloma (Rajkumaret al., 2014). Following hospitalization in October 2018, the patient opted for a complementary approach to her condition consisting in consumption of 50 mL, twice a day, of an extremely biodiverse probiotic drink obtained by fermentation of bovine milk and colostrum; in addition, the Medical Doctor supervising the patient, in October 2018 begun administering 0.2 mL per week of a novel immune-modulating supplement based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine (Ruggiero, 2018). A laboratory exam performed three months later (January 10, 2019) demonstrated a 95% decrease of serum free Kappa light chains that went from 2,190 mg/L (October 2018) to 119.40 mg/L. Also the ratio Kappa/Lambda showed a trend toward normalization decreasing from 187 (October 2018) to 11.10 (January 2019). Consistent with the significant improvement of the oncohematological picture, also the renal function improved, albeit to a lesser extent. Creatinine decreased from 1.76 mg/dL (October 2018) to 1.61 mg/dL (January 2019); glomerular filtrate increased from 28 mL/min (October 2018) to 34 mL/min (January 2019). Two months later (March 1, 2019) the patient was admitted to the Hospital of Eboli, Italy, for a trauma to her left leg due to an accidental fall at home; the laboratory exams performed on that occasion demonstrated further improvement of the oncohematological condition with the ratio Kappa/Lambda decreased to 3.5 that is very close to the upper limit of normal values (2.93). A further laboratory exam performed about two months later (May 24, 2019) at the University Hospital San Giovanni di Dio e Ruggi d’Aragona, Salerno, Italy, confirmed the continuing descending trend of the Kappa/Lambda ratio that was 3.16. Consistent with these results, a bone marrow aspiration at the sternum performed on April 26, 2019 at the San Giuseppe Moscati Hospital, Avellino, Italy, evidenced plasma cell infiltration of 5% that is a very significant reduction in comparison to the results observed before implementing the nutritional approach described above; thus, plasma cell bone marrow infiltration was 20% in October 2018. The report from the San Giuseppe Moscati Hospital was consistent with a quasi-normal

condition as it read: “Bone marrow aspiration. Slightly reduced bone density. Specimen very rich in cells and tiny lumps. Parenchymal erythroblasts and granulocytes are normal and in normal stage of maturation. Number of lymphocytes is normal. Very slight (in Italian, lievissimo) increase of plasma cells that are present in a percentage of about 5%; some plasma cell is binucleate. Megakaryocytes are numerous and most of them in active terminal thrombopoiesis.” (literal translation from Italian).

Discussion

A number of preclinical and clinical trials highlights the importance of the microbiota in cancer and there is general consensus that modulation of the microbiota plays a fundamental role in determining the response of cancer to therapies (Gopalakrishnan et al., 2018). The observation described in this case supports the hypothesis that a combination of a microbiota-based approach with immune-modulating strategies may prove effective in oncohematological diseases. It is worth noticing that the milk and colostrum fermented drink assumed by the patient here described represents a novel form of probiotic because of its extreme biodiversity; ingredients, microbial composition and nutritional characteristics of this drink are described in Table 1. A microbiome assay performed with a chip covered in DNA sequences that are specific to target organisms and is able to detect a total of approximately 12,000 species (Thissen et al., 2019), revealed the presence of hundreds of different microorganisms, thus showing an extremely high degree of biodiversity that included plasmids and phages with the latter being heralded as the tools for the future of immunotherapy (Rehman et al., 2019). It is known that phages are endowed with anticancer activity and it was demonstrated that they inhibit the growth of experimental melanoma and lung cancer and also inhibit the formation of lung metastases (Dabrowska et al., 2004). In addition, phages are responsible for activation of tumoricidal, classically activated, M1 macrophages and it was demonstrated that, following cancer treatment with phages, the M2-polarized tumor microenvironment switched toward an M1-polarized milieu (Eriksson et al., 2009). At the molecular level, an example highlighting the role of the phages is represented by the presence ofLactococcus lactis phage ul36 (Siphoviridae) in the probiotic drink assumed by the patient. As demonstrated since 2008, this microorganism encodes a single-strand annealing protein (Sak) that is homologous to the human recombination protein RAD52, a protein that plays crucial roles in DNA repair and genome stability (Ploquin et al., 2008).

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Table 1: Characteristics of the probiotic

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Description of the product Ingredients, colostrum Ingredients, microbial blends

Nutritional information

Product based on bovine colostrum and live probiotics for home preparation of fermented milk.
Freeze-dried, pasteurized bovine colostrum. 5 grams per liter of final product.
Blend of lyophilized kefir grains containing live cultures (including Streptococcus thermophilus and Lactobacillus delbrueckii, subspecie bulgaricus) and yeasts.
Blend of Bifidobacteria: Bifidobacterium infantis (25%); Bifidobacterium bifidum (25%); Bifidobacterium lactis (25%); Bifidobacterium longum (25%).
Blend of lyophilized micro-encapsulated live cultures; Bifidobacterium infantis (12.5%); Bifidobacterium bifidum (12.5%); Bifidobacterium lactis (12.5%); Lactobacillus salivarius (12.5%); Lactobacillus acidophilus (12.5%); Lactobacillus casei, subspecie paracasei (12.5%); Lactobacillus rhamnosus (12.5%); Lactococcus lactis, subspecie cremoris (12.5%). Coating agents for this blend (micro-encapsulation); mono- and diglycerides of fatty acids. Calories: 4 Kcal. Proteins: 0.28 grams. Fats: 0.28 grams. Carbohydrates: 0.22 grams. These values refer to the ingredients listed above in the measure required to prepare 1 liter of final product.

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The ingredients listed above are used by the final utilizer to ferment bovine milk at room temperature for 24 h and prepare a probiotic drink. The microbiome assay described in the text refers to the product of fermentation and not to the individual ingredients listed above. The microbiome assay was performed by an independent laboratory on the fermented probiotic drink that was lyophilized prior to the assay. We hypothesize that the extreme biodiversity mentioned in the text is due to the process of fermentation as well as to the complex microflora of the kefir grains.

From an evolutionary perspective, however, it is the human RAD52 protein that has evolved from the phage Sak protein, thus further emphasizing the role of phage-associated genetic information in maintaining DNA stability and, in a broader sense, human health.

It is tempting to speculate that the wide-range of effects on the immune system deriving from the extreme biodiversity of the probiotic drink assumed by the patient was synergistic with the effects of the supplement based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine as they are described in two recent papers (Ruggiero, 2018; Ruggiero and Pacini, 2018). Such a synergism may well be at work at the molecular level, for example by integrating different mechanisms responsible for DNA repair that counteract DNA instability, a common trait of cancer and other conditions. On one hand, the Sak/RAD52 pathway of the probiotic targets single-stranded DNAs preferentially over double-stranded DNAs and promotes the renaturation of long complementary single-stranded DNAs (Ploquin et al., 2008); on the other hand, the vitamin D3 of the supplement promotes expression of the DNA repair genes RAD50 and ATM (Ataxia Telangiectasia Mutated), that play a key role in mediating the signaling responses to double-strand DNA breaks (Ting et al., 2012). The convergence of these pathways maximizes the ability of cells to restore single- and double-strand DNA breaks thus efficiently counteracting a variety of DNA damages that are considered responsible for cancer onset and progression. It is also worth emphasizing the synergism between the phages in the probiotic and the phosphatidylcholine in the supplement in promoting and stabilizing a strong M1 phenotype in macrophage polarization (Qin et al., 2014).

Since this clinical case report is an open-label, non- controlled, retrospective analysis, caution must be exercised when ascribing cause and effect to any

treatment outcome. In addition, as of June 2019, the patient is assuming the following drugs in the context of chronic renal disease management; prednisone, 5 mg/die; omeprazole, 20 mg/die; folic acid, 5 mg/die; febuxostat, 80 mg/die; telmisartan, 20 mg when needed; warfarin 5 mg administered according to the patient’s INR. The patient did not report side effects associated with the nutritional approaches described above assumed in conjunction with her current therapy. Although prednisone may have contributed to the observed results as it is used in the therapy of multiple myeloma, it is worth noticing that the patient had been assuming this drug alongside others for a long time. In addition, the dose for the management of multiple myeloma is ten times higher than that assumed by the patient (Berenson et al., 2002). Despite these limitations, it appears that the association between the probiotic and the supplement and the improvements demonstrated by objective laboratory data was robust. It is well accepted that case reports are constituents of evidence-based medicine and informed practice (Juyal et al., 2013) and we are convinced that this novel and unusual clinical observation may lead to subsequent research advances in the field of complementary immunotherapy of cancer.

Acknowledgements

The Authors wish to thank the patient whose case is described here and her relatives for their priceless collaboration.

Conflict of Interest

Nicola Antonucci is the founder of the Biomedical Centre for Autism Research and Treatment, a private clinic. Marco Ruggiero is the inventor of a number of supplements and, together with Stefania Pacini, developed the probiotic (Bravo, Silver Spring Sagl, ) and the supplement (imuno, imuno Corporation, Vanuatu) used in this study. Both the probiotic and the supplement were regularly purchased and paid for by the patient. Neither Dr. Ruggiero, nor Dr. Pacini, had any prior knowledge of the strategies being implemented nor of the details of the patient whose clinical outcomes were analyzed and described in this study. Marco Ruggiero is member of the Editorial Board of The American Journal of Immunology and is waived from the Article Processing fee for this contribution; he receives no remuneration for his editorial work.

Authors’ Contribution

Nicola Antonucci: Performed the therapeutic procedures described in this study and communicated the clinical and laboratory data of the patient.

Marco Ruggiero and Stefania Pacini: Wrote the first draft of this paper, provided critical input and assisted in revising and improving the paper.

Ethics

No information in this paper is presented by the authors as medical advice. Caregivers, researchers and interested parties should research all information given. Beginning any significant biomedical or other interventions that may impact physiology or making changes to an established regimen should be discussed with the patient’s physician in advance. Standard of care for each pathology must be followed as well as rules and regulations established by Health Authorities of each Country.

References

Rajkumar, S.V., 2016. Myeloma today: Disease definitions and treatment advances. Am. J. Hematol., 1: 90-100. DOI: 10.1002/ajh.24236

Eriksson, F., P. Tsagozis and K. Lundberg, R. Parsa and S.M. Mangsbo et al., 2009. Tumor-specific bacteriophages induce tumor destruction through activation of tumor-associated macrophages. J. Immunol., 182: 3105-3111.

DOI: 10.4049/jimmunol.0800224
Ploquin, M., A. Bransi and E.R. Paquet, A.Z. Stasiak and

A. Stasiak et al., 2008. Functional and structural basis for a bacteriophage homolog of human RAD52. Curr. Biol., 18: 1142-6.
DOI: 10.1016/j.cub.2008.06.071

Ruggiero, M. and S.A. Pacini, 2018. Novel potential adjuvant for cancer vaccines. Madridge J. Vaccines, 2: 57-1. DOI: 10.18689/mjv-1000112

Ting, H.J., S. Yasmin-Karim, S.J. Yan, J.W. Hsu and T.H. Lin et al., 2012. A positive feedback signaling loop between ATM and the vitamin D receptor is critical for cancer chemoprevention by vitamin D. Cancer Res., 72: 958-68.

DOI: 10.1158/0008-5472.CAN-11-0042
Qin, X., C. Qiu and L. Zhao, 2014. Lysophosphatidylcholine perpetuates macrophage polarization toward classically activated phenotype

in inflammation. Cell Immunol., 289: 185-90.

DOI: 10.1016/j.cellimm.2014.04.01015
Juyal, D., S. Thaledi and V. Thawani, 2013. Thawani writing patient case reports for publication. Educ.

Health, 26: 126-9. DOI: 10.4103/1357-6283.120707 Hungria, V.T.M., E.Q. Crusoé, R.I. Bittencourt, R.P. Magalhães and J.N. Sobrinho et al., 2019. New proteasome inhibitors in the treatment of multiple myeloma. Hematol. Transfus Cell Ther., 1: 76-83.

DOI: 10.1016/j.htct.2018.07.003
Mikhael, J., J. Manola, A.C. Dueck, S. Hayman and K.

Oettel et al., 2018. Lenalidomide and dexamethasone in patients with relapsed multiple myeloma and impaired renal function: PrE1003, a PrECOG study. Blood Cancer J.

DOI: 10.1038/s41408-018-0110-7
Rajkumar, S.V., M.A. Dimopoulos, A. Palumbo, J.

Blade and G. Merlini et al., 2014. International myeloma working group updated criteria for the diagnosis of multiple myeloma. Lancet Oncol,. 15: e538-48. DOI: 10.1016/S1470-2045(14)70442-5

Ruggiero, M., 2018. Rationale for the design of a novel tool for immunotherapy based on an emulsion of glycosaminoglycan. Integr. Cancer Sci. Therap, 5: 1-5. DOI: 10.15761/ICST.1000285

Gopalakrishnan, V., B.A. Helmink, C.N. Spencer, A. Reuben and J.A. Wargo et al., 2018. The Influence of the gut microbiome on cancer, immunity and cancer immunotherapy. Cancer Cell, 33: 570-580. DOI: 10.1016/j.ccell.2018.03.015

Thissen, J.B., N.A. Be, K. McLoughlin, S. Gardner and P.G. Rack et al., 2019. Axiom microbiome array, the next generation microarray for high-throughput pathogen and microbiome analysis. PLoS ONE. DOI: 10.1371/journal.pone.0212045

Rehman, S., Z. Ali, M. Khan, N. Bostan and S. Naseem et al., 2019. The dawn of phage therapy. Rev. Med. Virol. DOI: 10.1002/rmv.2041

Dabrowska, K., A. Opolski, J. Wietrzyk, K. Switala- Jelen and J. Boratynski et al., 2004. Antitumor activity of bacteriophages in murine experimental cancer models caused possibly by inhibition of 3 integrin signaling pathway. Acta Virol., 48: 241-248. PMID: 15745047

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Nicola Antonucci et al. / American Journal of Immunology 2019, ■■ (■): ■■■.■■■DOI: 10.3844/ajisp.2019.■■■.■■■

Berenson, J.R., J.J. Crowley, T.M. Grogan, J. Zangmeister and A.D. Briggs et al., 2002. Maintenance therapy with alternate-day prednisone improves survival in multiple myeloma patients. Blood, 99: 3163-8. DOI: 10.1182/blood.V99.9.3163

Accessory Material

Clinical and laboratory records are archived at the Biomedical Centre for Autism Research and Treatment, Bari. Italy. Since this is a low-number case report that does not produce generalizable knowledge, nor an investigation of an FDA regulated product, Institutional Review Board (IRB) review is not required for this activity (John Hopkins Medicine. 102.3 Organization Policy on Single Case Reports and Case Series. https://www.hopkinsmedicine.org/institutional_review_b oard/guidelines_policies/organization_policies/102_3.ht ml. Web page accessed June 10, 2019).

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