Hypocholoris Acid HOCI

Note from Mimi- what got my attention about this product is Dr. Klinghardt’s presentation in a workshop I attended.  He said that in England and Europe when there was a case of mad cow’s disease, they removed and burned the examination table and other contents.

Now they use hypochlorous acid.  He said it is gentle enough to use on eyelash mites.  (And it works with Covid 19 but his presentation was earlier.)

He sells it on his site.  But being who I am, I bought a machine to make it and put it in a spray bottle.  I clean rooms, air spray, and clean floors and outside furniture with it. 

I tried to put it in my pool but I would need a real chlorine generator to do that job.  I believe that this generator make this same ingredient.

Trouble free pools.com

Chlorine, and specifically the active chlorine compound hypochlorous acid (HOCl), is a very powerful oxidizer.

I bought this machine 

https://store.ecoloxtech.com/ecoone?sku=E-1&gclid=CjwKCAjwsfuYBhAZEiwA5a6CDGE8OBljPGiIIhxsjJfADlv2a8rkzDneYfL2dEfEFkZ4lH1WV5_3ARoC2RIQAvD_BwE

I gave my chefs a machine and they LOVE it.

In food service and water distribution, specialized equipment to generate weak solutions of HClO from water and salt is sometimes used to generate adequate quantities of safe (unstable) disinfectant to treat food preparation surfaces and water supplies.[19][20] It is also commonly used in restaurants due to its non-flammable and nontoxic characteristics.

Hypochlorous acid (HClO, HOCl, or ClHO

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Names
IUPAC namehypochlorous acid, chloric(I) acid, chloranol, hydroxidochlorine
Other namesHydrogen hypochlorite, Chlorine hydroxide, hypochloric acid
Identifiers
CAS Number7790-92-3 
3D model (JSmol)Interactive image
ChEBICHEBI:24757 
ChemSpider22757 
ECHA InfoCard100.029.302 
EC Number232-232-5
PubChem CID24341
UNII712K4CDC10 
CompTox Dashboard (EPA)DTXSID3036737 
showInChI
showSMILES
Properties
Chemical formulaHClO 
Molar mass52.46 g/mol 
AppearanceColorless aqueous solution
DensityVariable
Solubility in waterSoluble
Acidity (pKa)7.53[1]
Conjugate baseHypochlorite
Hazards
Occupational safety and health (OHS/OSH):
Main hazardscorrosive, oxidizing agent
NFPA 704(fire diamond)NFPA 704 four-colored diamond304OX
Related compounds
Other anionsHypofluorous acid
Hypobromous acid
Hypoiodous acid
Related compoundsChlorine
Calcium hypochlorite
Sodium hypochlorite
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).check verify (what is  ?)Infobox references

Hypochlorous acid (HClO, HOCl, or ClHO[2][3]) is a weak acid that forms when chlorine dissolves in water, and itself partially dissociates, forming hypochlorite, ClO. HClO and ClO are oxidizers, and the primary disinfection agents of chlorine solutions.[4] HClO cannot be isolated from these solutions due to rapid equilibration with its precursorchlorine.

Because of its strong antimicrobial properties, the related compounds sodium hypochlorite (NaClO) and calcium hypochlorite (Ca(ClO)2) are ingredients in many commercial bleachesdeodorants, and disinfectants.[5] The white blood cells of mammals, such as humans, also contain hypochlorous acid as a tool against foreign bodies.[6]

Like many other disinfectants, hypochlorous acid solutions will destroy pathogens, such as COVID-19, adsorbed on surfaces.[7] In low concentrations, such solutions can serve to disinfect open wounds.[8]

Contents

History[edit]

Hypochlorous acid was discovered in 1834 by the French chemist Antoine Jérôme Balard (1802–1876) by adding, to a flask of chlorine gas, a dilute suspension of mercury(II) oxide in water.[9] He also named the acid and its compounds.[10]

Despite being relatively easy to make, it is difficult to maintain a stable hypochlorous acid solution. It is not until recent years that scientists have been able to cost-effectively produce and maintain hypochlorous acid water for stable commercial use.

Uses[edit]

  • In organic synthesis, HClO converts alkenes to chlorohydrins.[11]
  • In biology, hypochlorous acid is generated in activated neutrophils by myeloperoxidase-mediated peroxidation of chloride ions, and contributes to the destruction of bacteria.[12][13][14]
  • In medicine, hypochlorous acid water has been used as a disinfectant and sanitiser.[6][8][5]
  • In wound care,[15][16][17] and as of early 2016 the U.S. Food and Drug Administration has approved products whose main active ingredient is hypochlorous acid for use in treating wounds and various infections in humans and pets. It is also FDA-approved as a preservative for saline solutions.
  • In disinfection, it has been used in the form of liquid spray, wet wipes and aerosolised application. Recent studies have shown hypochlorous acid water to be suitable for fog and aerosolised application for disinfection chambers and suitable for disinfecting indoor settings such as offices, hospitals and healthcare clinics.[18]
  • In food service and water distribution, specialized equipment to generate weak solutions of HClO from water and salt is sometimes used to generate adequate quantities of safe (unstable) disinfectant to treat food preparation surfaces and water supplies.[19][20] It is also commonly used in restaurants due to its non-flammable and nontoxic characteristics.
  • In water treatment, hypochlorous acid is the active sanitizer in hypochlorite-based products (e.g. used in swimming pools).[21]
  • Similarly, in ships and yachts, marine sanitation devices[22] use electricity to convert seawater into hypochlorous acid to disinfect macerated faecal waste before discharge into the sea.
  • In deodorization, hypochlorous acid has been tested to remove up to 99% of foul odours including garbage, rotten meat, toilet, stool, and urine odours.[citation needed]

Formation, stability and reactions[edit]

Addition of chlorine to water gives both hydrochloric acid (HCl) and hypochlorous acid (HClO):[23]Cl2 + H2O ⇌ HClO + HClCl2 + 4 OH ⇌ 2 ClO + 2 H2O + 2 eCl2 + 2 e ⇌ 2 Cl

When acids are added to aqueous salts of hypochlorous acid (such as sodium hypochlorite in commercial bleach solution), the resultant reaction is driven to the left, and chlorine gas is formed. Thus, the formation of stable hypochlorite bleaches is facilitated by dissolving chlorine gas into basic water solutions, such as sodium hydroxide.

The acid can also be prepared by dissolving dichlorine monoxide in water; under standard aqueous conditions, anhydrous hypochlorous acid is currently impossible to prepare due to the readily reversible equilibrium between it and its anhydride:[24]2 HClO ⇌ Cl2O + H2O      K (at 0 °C) = 3.55×10−3 dm3 mol−1

The presence of light or transition metal oxides of coppernickel, or cobalt accelerates the exothermic decomposition into hydrochloric acid and oxygen:[24]2 Cl2 + 2 H2O → 4 HCl + O2

Fundamental reactions[edit]

In aqueous solution, hypochlorous acid partially dissociates into the anion hypochlorite ClO:HClO ⇌ ClO + H+

Salts of hypochlorous acid are called hypochlorites. One of the best-known hypochlorites is NaClO, the active ingredient in bleach.

HClO is a stronger oxidant than chlorine under standard conditions.2 HClO(aq) + 2 H+ + 2 e ⇌ Cl2(g) + 2 H2O  E = +1.63 V

HClO reacts with HCl to form chlorine:HClO + HCl → H2O + Cl2

HClO reacts with ammonia to form monochloramine:NH3 + HClO → NH2Cl + H2O

HClO can also react with organic amines, forming N-chloroamines.

Hypochlorous acid exists in equilibrium with its anhydridedichlorine monoxide.[24]2 HClO ⇌ Cl2O + H2O       K (at 0 °C) = 3.55×10−3 dm3 mol−1

Reactivity of HClO with biomolecules[edit]

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Hypochlorous acid reacts with a wide variety of biomolecules, including DNARNA,[14][25][26][27] fatty acid groups, cholesterol[28][29][30][31][32][33][34][35] and proteins.[31][36][37][38][39][40][41]

Reaction with protein sulfhydryl groups[edit]

Knox et al.[39] first noted that HClO is a sulfhydryl inhibitor that, in sufficient quantity, could completely inactivate proteins containing sulfhydryl groups. This is because HClO oxidises sulfhydryl groups, leading to the formation of disulfide bonds[42] that can result in crosslinking of proteins. The HClO mechanism of sulfhydryl oxidation is similar to that of monochloramine, and may only be bacteriostatic, because once the residual chlorine is dissipated, some sulfhydryl function can be restored.[38] One sulfhydryl-containing amino acid can scavenge up to four molecules of HClO.[41]Consistent with this, it has been proposed that sulfhydryl groups of sulfur-containing amino acids can be oxidized a total of three times by three HClO molecules, with the fourth reacting with the α-amino group. The first reaction yields sulfenic acid (R–SOH) then sulfinic acid (R–SO2H) and finally R–SO3H. Sulfenic acids form disulfides with another protein sulfhydryl group, causing cross-linking and aggregation of proteins. Sulfinic acid and R–SO3H derivatives are produced only at high molar excesses of HClO, and disulfides are formed primarily at bacteriocidal levels.[27] Disulfide bonds can also be oxidized by HClO to sulfinic acid.[42] Because the oxidation of sulfhydryls and disulfides evolves hydrochloric acid,[27] this process results in the depletion HClO.

Reaction with protein amino groups[edit]

Hypochlorous acid reacts readily with amino acids that have amino group side-chains, with the chlorine from HClO displacing a hydrogen, resulting in an organic chloramine.[43] Chlorinated amino acids rapidly decompose, but protein chloramines are longer-lived and retain some oxidative capacity.[13][41] Thomas et al.[13] concluded from their results that most organic chloramines decayed by internal rearrangement and that fewer available NH2 groups promoted attack on the peptide bond, resulting in cleavage of the protein. McKenna and Davies[44] found that 10 mM or greater HClO is necessary to fragment proteins in vivo. Consistent with these results, it was later proposed that the chloramine undergoes a molecular rearrangement, releasing HCl and ammonia to form an aldehyde.[45] The aldehyde group can further react with another amino group to form a Schiff base, causing cross-linking and aggregation of proteins.[31]

Reaction with DNA and nucleotides[edit]

Hypochlorous acid reacts slowly with DNA and RNA as well as all nucleotides in vitro.[25][46] GMP is the most reactive because HClO reacts with both the heterocyclic NH group and the amino group. In similar manner, TMP with only a heterocyclic NH group that is reactive with HClO is the second-most reactive. AMP and CMP, which have only a slowly reactive amino group, are less reactive with HClO.[46] UMP has been reported to be reactive only at a very slow rate.[14][25] The heterocyclic NH groups are more reactive than amino groups, and their secondary chloramines are able to donate the chlorine.[27] These reactions likely interfere with DNA base pairing, and, consistent with this, Prütz[46] has reported a decrease in viscosity of DNA exposed to HClO similar to that seen with heat denaturation. The sugar moieties are nonreactive and the DNA backbone is not broken.[46] NADH can react with chlorinated TMP and UMP as well as HClO. This reaction can regenerate UMP and TMP and results in the 5-hydroxy derivative of NADH. The reaction with TMP or UMP is slowly reversible to regenerate HClO. A second slower reaction that results in cleavage of the pyridine ring occurs when excess HClO is present. NAD+ is inert to HClO.[27][46]

Reaction with lipids[edit]

Hypochlorous acid reacts with unsaturated bonds in lipids, but not saturated bonds, and the ClO ion does not participate in this reaction. This reaction occurs by hydrolysis with addition of chlorineto one of the carbons and a hydroxyl to the other. The resulting compound is a chlorohydrin.[28] The polar chlorine disrupts lipid bilayers and could increase permeability.[29] When chlorohydrin formation occurs in lipid bilayers of red blood cells, increased permeability occurs. Disruption could occur if enough chlorohydrin is formed.[28][34] The addition of preformed chlorohydrin to red blood cells can affect permeability as well.[30] Cholesterol chlorohydrin have also been observed,[29][32] but do not greatly affect permeability, and it is believed that Cl2 is responsible for this reaction.[32]

Mode of disinfectant action[edit]

E. coli exposed to hypochlorous acid lose viability in less than 0.1 seconds due to inactivation of many vital systems.[23][47][48][49][50] Hypochlorous acid has a reported LD50 of 0.0104–0.156 ppm[51]and 2.6 ppm caused 100% growth inhibition in 5 minutes.[44] However, the concentration required for bactericidal activity is also highly dependent on bacterial concentration.[39]

Inhibition of glucose oxidation[edit]

In 1948, Knox et al.[39] proposed the idea that inhibition of glucose oxidation is a major factor in the bacteriocidal nature of chlorine solutions. They proposed that the active agent or agents diffuse across the cytoplasmic membrane to inactivate key sulfhydryl-containing enzymes in the glycolytic pathway. This group was also the first to note that chlorine solutions (HClO) inhibit sulfhydrylenzymes. Later studies have shown that, at bacteriocidal levels, the cytosol components do not react with HClO.[52] In agreement with this, McFeters and Camper[53] found that aldolase, an enzyme that Knox et al.[39] proposes would be inactivated, was unaffected by HClO in vivo. It has been further shown that loss of sulfhydryls does not correlate with inactivation.[38] That leaves the question concerning what causes inhibition of glucose oxidation. The discovery that HClO blocks induction of β-galactosidase by added lactose[54] led to a possible answer to this question. The uptake of radiolabeled substrates by both ATP hydrolysis and proton co-transport may be blocked by exposure to HClO preceding loss of viability.[52] From this observation, it proposed that HClO blocks uptake of nutrients by inactivating transport proteins.[37][52][53][55] The question of loss of glucose oxidation has been further explored in terms of loss of respiration. Venkobachar et al.[56]found that succinic dehydrogenase was inhibited in vitro by HClO, which led to the investigation of the possibility that disruption of electron transport could be the cause of bacterial inactivation. Albrich et al.[14] subsequently found that HClO destroys cytochromes and iron-sulfur clusters and observed that oxygen uptake is abolished by HClO and adenine nucleotides are lost. It was also observed that irreversible oxidation of cytochromes paralleled the loss of respiratory activity. One way of addressing the loss of oxygen uptake was by studying the effects of HClO on succinate-dependent electron transport.[57] Rosen et al.[50] found that levels of reductable cytochromes in HClO-treated cells were normal, and these cells were unable to reduce them. Succinate dehydrogenase was also inhibited by HClO, stopping the flow of electrons to oxygen. Later studies[48] revealed that Ubiquinol oxidase activity ceases first, and the still-active cytochromes reduce the remaining quinone. The cytochromes then pass the electrons to oxygen, which explains why the cytochromes cannot be reoxidized, as observed by Rosen et al.[50] However, this line of inquiry was ended when Albrich et al.[36] found that cellular inactivation precedes loss of respiration by using a flow mixing system that allowed evaluation of viability on much smaller time scales. This group found that cells capable of respiring could not divide after exposure to HClO.

Depletion of adenine nucleotides[edit]

Having eliminated loss of respiration, Albrich et al.[36] proposes that the cause of death may be due to metabolic dysfunction caused by depletion of adenine nucleotides. Barrette et al.[54] studied the loss of adenine nucleotides by studying the energy charge of HClO-exposed cells and found that cells exposed to HClO were unable to step up their energy charge after addition of nutrients. The conclusion was that exposed cells have lost the ability to regulate their adenylate pool, based on the fact that metabolite uptake was only 45% deficient after exposure to HClO and the observation that HClO causes intracellular ATP hydrolysis. It was also confirmed that, at bacteriocidal levels of HClO, cytosolic components are unaffected. So it was proposed that modification of some membrane-bound protein results in extensive ATP hydrolysis, and this, coupled with the cells inability to remove AMP from the cytosol, depresses metabolic function. One protein involved in loss of ability to regenerate ATP has been found to be ATP synthetase.[37] Much of this research on respiration reconfirms the observation that relevant bacteriocidal reactions take place at the cell membrane.[37][54][58]

Inhibition of DNA replication[edit]

Recently it has been proposed that bacterial inactivation by HClO is the result of inhibition of DNA replication. When bacteria are exposed to HClO, there is a precipitous decline in DNA synthesisthat precedes inhibition of protein synthesis, and closely parallels loss of viability.[44][59] During bacterial genome replication, the origin of replication (oriC in E. coli) binds to proteins that are associated with the cell membrane, and it was observed that HClO treatment decreases the affinity of extracted membranes for oriC, and this decreased affinity also parallels loss of viability. A study by Rosen et al.[60] compared the rate of HClO inhibition of DNA replication of plasmids with different replication origins and found that certain plasmids exhibited a delay in the inhibition of replication when compared to plasmids containing oriC. Rosen’s group proposed that inactivation of membrane proteins involved in DNA replication are the mechanism of action of HClO.

Protein unfolding and aggregation[edit]

HClO is known to cause post-translational modifications to proteins, the notable ones being cysteine and methionine oxidation. A recent examination of HClO’s bactericidal role revealed it to be a potent inducer of protein aggregation.[61] Hsp33, a chaperone known to be activated by oxidative heat stress, protects bacteria from the effects of HClO by acting as a holdase, effectively preventing protein aggregation. Strains of Escherichia coli and Vibrio cholerae lacking Hsp33 were rendered especially sensitive to HClO. Hsp33 protected many essential proteins from aggregation and inactivation due to HClO, which is a probable mediator of HClO’s bactericidal effects.

Hypochlorites[edit]

Main article: Hypochlorite

Hypochlorites are the salts of hypochlorous acid; commercially important hypochlorites are calcium hypochlorite and sodium hypochlorite.

Production of hypochlorites using electrolysis[edit]

See also: Chloralkali process

Solutions of hypochlorites can be produced in-situ by electrolysis of an aqueous sodium chloride solution in both batch and flow processes.[62] The composition of the resulting solution depends on the pH at the anode. In acid conditions the solution produced will have a high hypochlorous acid concentration, but will also contain dissolved gaseous chlorine, which can be corrosive, at a neutral pH the solution will be around 75% hypochlorous acid and 25% hypochlorite. Some of the chlorine gas produced will dissolve forming hypochlorite ions. Hypochlorites are also produced by the disproportionation of chlorine gas in alkaline solutions.

Safety[edit]

HClO is classified as Non-Hazardous by the Environmental Protection Agency in the US. As any oxidising agent it can be corrosive or irritant depending on its concentration and pH.

In a clinical test, hypochlorous acid water was tested for eye irritation, skin irritation, and toxicity. The test concluded that it was non-toxic and nonirritating to the eye and skin.[63]

In a 2017 study, a saline hygiene solution preserved with pure hypochlorous acid was shown to reduce the bacterial load significantly without altering the diversity of bacterial species on the eyelids. After 20 minutes of treatment, there was >99% reduction of the Staphylococci bacteria.[64]

Commercialisation[edit]

For disinfection, despite being discovered a long time ago, the stability of hypochlorous acid water is difficult to maintain. In solution, the active compounds quickly deteriorate back into salt water, losing its disinfecting capability, which makes it difficult to transport for wide use. Despite its stronger disinfecting capabilities, it is less commonly used as a disinfectant compared to bleach and alcohol due to cost.

Technological developments have reduced manufacturing costs and allow for manufacturing and bottling of hypochlorous acid water for home and commercial use. However, most hypochlorous acid water has a short shelf life. Storing away from heat and direct sunlight can help slow the deterioration. The further development of continuous flow electrochemical cells has been implemented in new products, allowing the commercialisation of domestic and industrial continuous flow devices for the in-situ generation of hypochlorous acid for disinfection purposes.[65]

See also[edit]

References[edit]

  1. ^ Harris, Daniel C. (2009). Exploring Chemical Analysis(Fourth ed.). p. 538.
  2. ^ “Hypochlorous acid”CAS Common ChemistryCAS, a division of the American Chemical Society, n.d. CAS RN: 7790-92-3. Retrieved 2022-04-12.
  3. ^ “hypochlorous acid”Chemical Entities of Biological InterestEuropean Bioinformatics Institute. CHEBI:24757. Retrieved 2022-04-12.
  4. ^ Sansebastiano, G. et al. Page 262 in Food Safety: A Practical and Case Study Approach (Ed: R. J. Marshall) 2006, Springer Science & Business Media, Berlin.
  5. Jump up to: a b Block, Michael S.; Rowan, Brian G. (September 2020). “Hypochlorous Acid: A Review”Journal of Oral and Maxillofacial Surgery78 (9): 1461–1466. doi:10.1016/j.joms.2020.06.029ISSN 0278-2391PMC 7315945PMID 32653307.
  6. Jump up to: a b “Treating Chronic Wounds With Hypochlorous Acid Disrupts Biofilm”Today’s Wound Clinic. Retrieved 2021-02-08.
  7. ^ US EPA, OCSPP (2020-03-13). “List N: Disinfectants for Coronavirus (COVID-19)”US EPA. Retrieved 2021-02-08.
  8. Jump up to: a b “Pure Hypochlorous Acid: A Primer on pH and Wound Solutions”WoundSource. 2020-11-05. Retrieved 2021-02-08..
  9. ^ See:
    • Balard, A. J. (1834). “Recherches sur la nature des combinaisons décolorantes du chlore” [Investigations into the nature of bleaching compounds of chlorine]. Annales de Chimie et de Physique. 2nd series (in French). 57: 225–304. From p. 246:  ” … il est beaucoup plus commode … environ d’eau distillée.” ( … it is much easier to pour, into flasks full of chlorine, red mercury oxide [that has been] reduced to a fine powder by grinding and diluted in about twelve times its weight of distilled water.)
    • Graham, Thomas (1840). Elements of Chemistry. Vol. 4. London, England: H. Baillière. p. 367.
  10. ^ (Balard, 1834), p. 293. From p. 293:  “Quelle dénomination … appelées hypochlorites.” (What name should one assign to this compound? It’s obvious that that of “chlorous acid” can hardly be retained for it, and that it is more appropriate to call it hypochlorous acid, a name that recalls its similarity of composition with hyposulfurous acid, hypophosphorous acid, etc., [which are] formed, like it, from 1 equivalent of their radical and 1 equivalent of oxygen. Its compounds will be called hypochlorites.)
  11. ^ Unangst, P. C. “Hypochlorous Acid” in Encyclopedia of Reagents for Organic Synthesis (Ed: L. Paquette) 2004, J. Wiley & Sons, New York. doi:10.1002/047084289X.rh073
  12. ^ Harrison, J. E.; J. Schultz (1976). “Studies on the chlorinating activity of myeloperoxidase”Journal of Biological Chemistry251 (5): 1371–1374. doi:10.1016/S0021-9258(17)33749-3PMID 176150.
  13. Jump up to: a b c Thomas, E. L. (1979). “Myeloperoxidase, hydrogen peroxide, chloride antimicrobial system: Nitrogen-chlorine derivatives of bacterial components in bactericidal action against Escherichia coliInfect. Immun23 (2): 522–531. doi:10.1128/IAI.23.2.522-531.1979PMC 414195PMID 217834.
  14. Jump up to: a b c d Albrich, J. M., C. A. McCarthy, and J. K. Hurst (1981). “Biological reactivity of hypochlorous acid: Implications for microbicidal mechanisms of leukocyte myeloperoxidase”Proc. Natl. Acad. Sci78 (1): 210–214. Bibcode:1981PNAS…78..210Adoi:10.1073/pnas.78.1.210PMC 319021PMID 6264434.
  15. ^ Wang L et al. “Hypochlorous acid as a potential wound care agent. Part I Stabilized hypochlorous acid: a component of the inorganic armamentarium of innate immunity”. J Burns and Wounds 2007; April: 65–79.
  16. ^ Robson MC et al. “Hypochlorous acid as a potential wound care agent. Part II Stabilized hypochlorous acid: its role in decreasing tissue bacterial bioburden and overcoming the inhibition of infection on wound healing”Journal of Burns and Wounds 2007; April: 80–90.
  17. ^ Selkon, JB; et al. (2006). “Evaluation of hypochlorous acid washes in the treatment of venous leg ulcers”. J Wound Care2006 (15): 33–37. doi:10.12968/jowc.2006.15.1.26861PMID 16669304.
  18. ^ Nguyen, Kate; Bui, Dinh; Hashemi, Mahak; Hocking, Dianna M; Mendis, Priyan; Strugnell, Richard A; Dharmage, Shyamali C (2021-01-22). “The Potential Use of Hypochlorous Acid and a Smart Prefabricated Sanitising Chamber to Reduce Occupation-Related COVID-19 Exposure”Risk Management and Healthcare Policy14: 247–252. doi:10.2147/RMHP.S284897ISSN 1179-1594PMC 7837568PMID 33519249.
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  20. ^ “Water Works: Hyatt’s New Disinfectant/Cleaner Comes from the Tap”Bloomberg Businessweek.
  21. ^ Gonick, Larry; Criddle, Craig (2005-05-03). “Chapter 9 Acid Basics”The cartoon guide to chemistry (1st ed.). HarperResource. p. 189ISBN 9780060936778Similarly, we add HOCl to swimming pools to kill bacteria.
  22. ^ e.g. Raritan Electro Scan device
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  24. Jump up to: a b c Inorganic chemistry, Egon Wiberg, Nils Wiberg, Arnold Frederick Holleman, “Hypochlorous acid”, p. 442, section 4.3.1
  25. Jump up to: a b c Dennis, W. H., Jr, V. P. Olivieri, and C. W. Krusé (1979). “The reaction of nucleotides with aqueous hypochlorous acid”. Water Res13 (4): 357–362. doi:10.1016/0043-1354(79)90023-X.
  26. ^ Jacangelo, J. G., and V. P. Olivieri. 1984. Aspects of the mode of action of monochloramine. In R. L. Jolley, R. J. Bull, W. P. Davis, S. Katz, M. H. Roberts, Jr., and V. A. Jacobs (ed.), Water Chlorination, vol. 5. Lewis Publishers, Inc., Williamsburg.
  27. Jump up to: a b c d e Prütz, WA (1998). “Interactions of hypochlorous acid with pyrimidine nucleotides, and secondary reactions of chlorinated pyrimidines with GSH, NADH, and other substrates”. Archives of Biochemistry and Biophysics349(1): 183–91. doi:10.1006/abbi.1997.0440PMID 9439597.
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